International Immunology, Vol 10, 1981-1985, Copyright © 1998 by Oxford University Press
N Takemoto, N Koyano-Nakagawa, T Yokota, N Arai, S Miyatake and K Arai
IL-4 and IL-13 are cytokines preferentially produced by Th2 cells, and
their genes are located in close proximity on human chromosome 5 and mouse
chromosome 11. To identify potential regulatory elements that confer
Th2-specific expression of IL-4 and IL-13 genes, we constructed a physical
map of the IL-13/IL-4 locus and conducted DNase I- hypersensitive (DH) site
analysis using Th clones and in vitro- differentiated effector Th cells
obtained from TCR transgenic mice. Three DH sites, HSS1, HSS2 and HSS3,
were identified within the intergenic region between IL-13 and IL-4 genes.
HSS3 was observed both in Th1 and Th2 cells as well as CD4+ naive T cells,
while HSS1 and HSS2 were detected exclusively in Th2 cells. The correlation
between differentiation into Th2 subtype and the appearance of HSS1 and
HSS2 suggests that these regions may play a role in subtype-specific
expression of the IL-13/IL-4 locus.
ARTICLES
Th2-specific DNase I-hypersensitive sites in the murine IL-13 and IL-4 intergenic region
Department of Molecular and Developmental Biology, The Institute of Medical Science, The University of Tokyo, Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  X. Zhao, B. Zheng, Y. Huang, D. Yang, S. Katzman, C. Chang, D. Fowell, and W.-p. Zeng Interaction between GATA-3 and the Transcriptional Coregulator Pias1 Is Important for the Regulation of Th2 Immune Responses J. Immunol., December 15, 2007; 179(12): 8297 - 8304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Yagi, S. Tanaka, Y. Motomura, and M. Kubo Regulation of the Il4 Gene Is Independently Controlled by Proximal and Distal 3' Enhancers in Mast Cells and Basophils Mol. Cell. Biol., December 1, 2007; 27(23): 8087 - 8097. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. T. Khaled, E. K. C. Read, S. E. Nicholson, F. O. Baxter, A. J. Brennan, P. J. Came, N. Sprigg, A. N. J. McKenzie, and C. J. Watson The IL-4/IL-13/Stat6 signalling pathway promotes luminal mammary epithelial cell development Development, August 1, 2007; 134(15): 2739 - 2750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhou, A. A. Nazarian, J. Xu, D. Tantin, L. M. Corcoran, and S. T. Smale An Inducible Enhancer Required for Il12b Promoter Activity in an Insulated Chromatin Environment Mol. Cell. Biol., April 1, 2007; 27(7): 2698 - 2712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Rose, M. Lichtenheld, M. R. Foote, and B. Adkins Murine Neonatal CD4+ Cells Are Poised for Rapid Th2 Effector-Like Function J. Immunol., March 1, 2007; 178(5): 2667 - 2678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Strempel and D. Vercelli Functional Dissection Identifies a Conserved Noncoding Sequence-1 Core That Mediates IL13 and IL4 Transcriptional Enhancement J. Biol. Chem., February 9, 2007; 282(6): 3738 - 3746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Webster, Y. Rodriguez, W. T. Klimecki, and D. Vercelli The Human IL-13 Locus in Neonatal CD4+ T Cells Is Refractory to the Acquisition of a Repressive Chromatin Architecture J. Biol. Chem., January 5, 2007; 282(1): 700 - 709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hayashida, M. Oda, K. Ohsawa, A. Yamaguchi, T. Hosozawa, R. M. Locksley, M. Giacca, H. Masai, and S. Miyatake Replication Initiation from a Novel Origin Identified in the Th2 Cytokine Cluster Locus Requires a Distant Conserved Noncoding Sequence J. Immunol., May 1, 2006; 176(9): 5446 - 5454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Baguet, X. Sun, T. Arroll, A. Krumm, and M. Bix Intergenic Transcription Is Not Required in Th2 Cells to Maintain Histone Acetylation and Transcriptional Permissiveness at the Il4-Il13 Locus J. Immunol., December 15, 2005; 175(12): 8146 - 8153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Monticelli, D. U. Lee, J. Nardone, D. L. Bolton, and A. Rao Chromatin-based regulation of cytokine transcription in Th2 cells and mast cells Int. Immunol., November 1, 2005; 17(11): 1513 - 1524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Koyanagi, A. Baguet, J. Martens, R. Margueron, T. Jenuwein, and M. Bix EZH2 and Histone 3 Trimethyl Lysine 27 Associated with Il4 and Il13 Gene Silencing in TH1 Cells J. Biol. Chem., September 9, 2005; 280(36): 31470 - 31477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Reiner Epigenetic control in the immune response Hum. Mol. Genet., April 15, 2005; 14(suppl_1): R41 - R46. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-Y. Wang, H. Sato, S. Kusam, S. Sehra, L. M. Toney, and A. L. Dent Regulation of IL-10 Gene Expression in Th2 Cells by Jun Proteins J. Immunol., February 15, 2005; 174(4): 2098 - 2105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. U. Lee and A. Rao Molecular analysis of a locus control region in the T helper 2 cytokine gene cluster: A target for STAT6 but not GATA3 PNAS, November 9, 2004; 101(45): 16010 - 16015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Im, A. Hueber, S. Monticelli, K.-H. Kang, and A. Rao Chromatin-level Regulation of the IL10 Gene in T Cells J. Biol. Chem., November 5, 2004; 279(45): 46818 - 46825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shnyreva, W. M. Weaver, M. Blanchette, S. L. Taylor, M. Tompa, D. R. Fitzpatrick, and C. B. Wilson Evolutionarily conserved sequence elements that positively regulate IFN-{gamma} expression in T cells PNAS, August 24, 2004; 101(34): 12622 - 12627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Baguet and M. Bix Chromatin landscape dynamics of the Il4-Il13 locus during T helper 1 and 2 development PNAS, August 3, 2004; 101(31): 11410 - 11415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Seki, M. Miyazaki, W. Suzuki, K. Hayashi, K. Arima, E. Myburgh, K. Izuhara, F. Brombacher, and M. Kubo IL-4-Induced GATA-3 Expression Is a Time-Restricted Instruction Switch for Th2 Cell Differentiation J. Immunol., May 15, 2004; 172(10): 6158 - 6166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cote-Sierra, G. Foucras, L. Guo, L. Chiodetti, H. A. Young, J. Hu-Li, J. Zhu, and W. E. Paul Interleukin 2 plays a central role in Th2 differentiation PNAS, March 16, 2004; 101(11): 3880 - 3885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Rogan, D. J. Cousins, S. Santangelo, P. A. Ioannou, M. Antoniou, T. H. Lee, and D. Z. Staynov Analysis of intergenic transcription in the human IL-4/IL-13 gene cluster PNAS, February 24, 2004; 101(8): 2446 - 2451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. U. Lee, O. Avni, L. Chen, and A. Rao A Distal Enhancer in the Interferon-{gamma} (IFN-{gamma}) Locus Revealed by Genome Sequence Comparison J. Biol. Chem., February 6, 2004; 279(6): 4802 - 4810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Adkins, T. Williamson, P. Guevara, and Y. Bu Murine Neonatal Lymphocytes Show Rapid Early Cell Cycle Entry and Cell Division J. Immunol., May 1, 2003; 170(9): 4548 - 4556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Soutto, W. Zhou, and T. M. Aune Cutting Edge: Distal Regulatory Elements Are Required to Achieve Selective Expression of IFN-{gamma} in Th1/Tc1 Effector Cells J. Immunol., December 15, 2002; 169(12): 6664 - 6667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yamashita, M. Ukai-Tadenuma, M. Kimura, M. Omori, M. Inami, M. Taniguchi, and T. Nakayama Identification of a Conserved GATA3 Response Element Upstream Proximal from the Interleukin-13 Gene Locus J. Biol. Chem., October 25, 2002; 277(44): 42399 - 42408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Takemoto, K.-i. Arai, and S. Miyatake Cutting Edge: The Differential Involvement of the N-Finger of GATA-3 in Chromatin Remodeling and Transactivation During Th2 Development J. Immunol., October 15, 2002; 169(8): 4103 - 4107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Santangelo, D. J. Cousins, N. E. E. Winkelmann, and D. Z. Staynov DNA Methylation Changes at Human Th2 Cytokine Genes Coincide with DNase I Hypersensitive Site Formation During CD4+ T Cell Differentiation J. Immunol., August 15, 2002; 169(4): 1893 - 1903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Guo, J. Hu-Li, J. Zhu, C. J. Watson, M. J. Difilippantonio, C. Pannetier, and W. E. Paul In TH2 cells the Il4 gene has a series of accessibility states associated with distinctive probabilities of IL-4 production PNAS, August 6, 2002; 99(16): 10623 - 10628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kurata, H.-J. Lee, T. McClanahan, R. L. Coffman, A. O'Garra, and N. Arai Friend of GATA Is Expressed in Naive Th Cells and Functions As a Repressor of GATA-3-Mediated Th2 Cell Development J. Immunol., May 1, 2002; 168(9): 4538 - 4545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cakouros, P. N. Cockerill, A. G. Bert, R. Mital, D. C. Roberts, and M. F. Shannon A NF-{{kappa}}B/Sp1 Region Is Essential for Chromatin Remodeling and Correct Transcription of a Human Granulocyte- Macrophage Colony-Stimulating Factor Transgene J. Immunol., July 1, 2001; 167(1): 302 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Z. Ben-Sasson, R. Gerstel, J. Hu-Li, and W. E. Paul Cell Division Is Not a ""Clock"" Measuring Acquisition of Competence to Produce IFN-{{gamma}} or IL-4 J. Immunol., January 1, 2001; 166(1): 112 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Takemoto, Y. Kamogawa, H. Jun Lee, H. Kurata, K.-i. Arai, A. O'Garra, N. Arai, and S. Miyatake Cutting Edge: Chromatin Remodeling at the IL-4/IL-13 Intergenic Regulatory Region for Th2-Specific Cytokine Gene Cluster J. Immunol., December 15, 2000; 165(12): 6687 - 6691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Kelly and R. M. Locksley Coordinate Regulation of the IL-4, IL-13, and IL-5 Cytokine Cluster in Th2 Clones Revealed by Allelic Expression Patterns J. Immunol., September 15, 2000; 165(6): 2982 - 2986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Lee, N. Takemoto, H. Kurata, Y. Kamogawa, S. Miyatake, A. O'Garra, and N. Arai GATA-3 Induces T Helper Cell Type 2 (Th2) Cytokine Expression and Chromatin Remodeling in Committed Th1 Cells J. Exp. Med., July 3, 2000; 192(1): 105 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Lacy, Z.-E. Wang, D. J. Symula, C. J. McArthur, E. M. Rubin, K. A. Frazer, and R. M. Locksley Faithful Expression of the Human 5q31 Cytokine Cluster in Transgenic Mice J. Immunol., May 1, 2000; 164(9): 4569 - 4574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. G. Loots, R. M. Locksley, C. M. Blankespoor, Z. E. Wang, W. Miller, E. M. Rubin, and K. A. Frazer Identification of a Coordinate Regulator of Interleukins 4, 13, and 5 by Cross-Species Sequence Comparisons Science, April 7, 2000; 288(5463): 136 - 140. [Abstract] [Full Text]
|
|
|
|
|
|
A. Richter, M. Lohning, and A. Radbruch Instruction for Cytokine Expression in T Helper Lymphocytes in Relation to Proliferation and Cell Cycle Progression J. Exp. Med., November 15, 1999; 190(10): 1439 - 1450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Guo, V. Casolaro, E. Seto, W.-M. Yang, C. Chang, M.-C. Seminario, J. Keen, and S. N. Georas Yin-Yang 1 Activates Interleukin-4 Gene Expression in T Cells J. Biol. Chem., December 21, 2001; 276(52): 48871 - 48878. [Abstract] [Full Text] [PDF]
|
|