International Immunology Advance Access published online on July 2, 2007
International Immunology, doi:10.1093/intimm/dxm061
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Response of human dendritic cells to different immunomodulatory polysaccharides derived from mushroom and barley
1 Department of Paediatrics and Adolescent Medicine, Hong Kong Jockey Club Clinical Research Center, Faculty of Medicine, University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong, China
2 Department of Pharmacology, Peking University Health Science Center, School of Basic Medical Sciences, Beijing, China
Correspondence to: Correspondence to: G. C. Chan; E-mail: gcfchan{at}hkucc.hku.hk
Polysaccharides derived from fungi and plants have been increasingly used as dietary supplement with therapeutic intention for cancer. However, whether these polysaccharides from different sources and structures can elicit similar immunological effects remain unknown. This study aims to investigate and compare the effects of selected groups of purified and crude polysaccharides on human dendritic cells (DCs), the most potent antigen-presenting cells. The selected polysaccharides were from Ganoderma lucidum [(GL) Lingzhi, Reishi], a medicinal mushroom commonly used by oriental; and barley glucan, a purified polysaccharide with known in vivo immunomodulating effect. We found that purified polysaccharides from GL mycelium could induce human PBMC proliferation and phenotypic and functional maturation of DCs with significant IL-12 and IL-10 production. Polysaccharides of GL spore and barley were both rather weak immunostimulator in vitro. In general, all these polysaccharides did not polarize T cells into either Th1 or Th2 or regulatory T cells, except for crude spore polysaccharides-treated DCs which could suppress T cell proliferation with IL-10 production. This study revealed the polysaccharides of different sources have different immune potency and effect on human immune cells including DCs. Our study also provides a reproducible biological platform for comparing the potential therapeutic effects of different herbal-derived polysaccharides in the future.
Keywords: barley, Ganoderma lucidum, human dendritic cell, immunomodulation, polysaccharides
Transmitting editor: J. Allison
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T.-L. Hsu, S.-C. Cheng, W.-B. Yang, S.-W. Chin, B.-H. Chen, M.-T. Huang, S.-L. Hsieh, and C.-H. Wong Profiling Carbohydrate-Receptor Interaction with Recombinant Innate Immunity Receptor-Fc Fusion Proteins J. Biol. Chem., December 11, 2009; 284(50): 34479 - 34489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Duan, F. Y. Avci, and D. L. Kasper Microbial carbohydrate depolymerization by antigen-presenting cells: Deamination prior to presentation by the MHCII pathway PNAS, April 1, 2008; 105(13): 5183 - 5188. [Abstract] [Full Text] [PDF]
|
|