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International Immunology Advance Access published online on August 16, 2007
International Immunology, doi:10.1093/intimm/dxm054
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© The Japanese Society for Immunology. 2007. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Ifi202, an IFN-inducible candidate gene for lupus susceptibility in NZB/W F1 mice, is a positive regulator for NF-{kappa}B activation in dendritic cells

Moriyasu Yamauchi1,2, Masayuki Hashimoto1, Kenji Ichiyama1, Ryoko Yoshida1, Toshikatsu Hanada1, Tatsushi Muta3, Shizuo Komune2, Takashi Kobayashi1 and Akihiko Yoshimura1

1 Division of Molecular and Cellular Immunology, Medical Institute of Bioregulation
2 Department of Otorhinolaryngology, Graduate School of Medical Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
3 Laboratory of Cell Recognition and Response, Graduate School of Life Science, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan

Correspondence to: Correspondence to: A. Yoshimura; E-mail: yakihiko{at}bioreg.kyushu-u.ac.jp

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the presence of autoantibodies and lupus nephritis. The [New Zealand black (NZB) x New Zealand white (NZW)]F1 (BWF1) mouse has been recognized as an important animal model of human SLE. The Th1-prone phenotype of BWF1 mice has been shown to contribute to the development of the lupus. However, the molecular basis for Th1 skewing in BWF1 mice has not been clarified. We noticed that IL-6, IL-12 and other proinflammatory cytokines as well as I{kappa}B-{zeta} induction were higher in mature bone marrow-derived dendritic cells (BMDCs) from NZB and BWF1 mice than those from NZW mice. The expression of an IFN-inducible gene Ifi202, a candidate gene for lupus, was almost undetectable in NZW BMDCs. Thus, we hypothesized that Ifi202 is involved in elevated IL-12 production from BWF1 BMDCs. Overexpression of Ifi202 enhanced the LPS-induced I{kappa}B-{zeta}, IL-12p40 and NF-{kappa}B promoter activities, while anti-sense (AS) RNA against Ifi202 strongly suppressed them in a monocytic cell line, RAW 264.7. Furthermore, overexpression of Ifi202 enhanced LPS-induced IL-12p40 and I{kappa}B-{zeta} mRNA induction while Ifi202 AS RNA suppressed these in RAW 264.7 cells. In addition, forced expression of Ifi202 enhanced IL-12p40 mRNA induction in NZW BMDCs. Thus, Ifi202 is an important NF-{kappa}B activator in DCs and involved in IL-12 production, which may account for a Th1-prone phenotype of BWF1 mice.

Keywords: dendritic cell, IL-12, interferon, SLE

Transmitting editor: T. Watanabe

Received 24 March 2007, accepted 24 April 2007.


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