International Immunology, Vol 9, 1271-1279, Copyright © 1997 by Oxford University Press
A Poggi, E Tomasello, V Revello, L Nanni, P Costa and L Moretta
p40 was previously described as a regulatory molecule capable of inhibiting
both the natural and the CD16-mediated cytotoxicity of NK cells. In this
study, we analyze the effect of p40 molecule engagement on the NK cell
triggering induced by activating HLA class I-specific NK receptors (NKR) or
on TCR alpha beta-mediated T cell activation. CD3- CD16+ NK cell clones
expressing activating NKR (either CD94 or p50) were analyzed in a
redirected killing assay using P815 target cells and appropriate mAb. A
strong target cell lysis was detected in the presence of anti-NKR or
anti-CD16 mAb alone. Addition of anti-p40 mAb resulted in a strong
inhibition of both anti-NKR or anti-CD16 mAb- induced cytolysis. mAb
specific for either CD45 or lymphocyte function associated antigen-1 did
not exert any inhibitory effect in the same experimental system. Free
intracellular calcium ([Ca2+]i) increase induced by mAb cross-linking of
activating CD94 or p50 was inhibited by simultaneous engagement of p40
molecules, but not of other NK surface molecules including CD44 and CD56.
In addition, cross-linking of p40 molecules strongly inhibited the
CD94-induced tumor necrosis factor- alpha and IFN-gamma production.
Analysis of TCR alpha beta or gamma delta T cell clones revealed that the
engagement of p40 molecules, using specific mAb, induced some degree of
inhibition only on anti-V beta (but not anti-V delta or anti-CD3)
mAb-induced cytotoxicity. On the other hand, the p40 molecule engagement
prevented T cell proliferation induced by either anti-V beta 8 or anti-V
delta 2 mAb. A similar inhibitory effect was found on the IL-2-induced NK
cell proliferation. Taken together, our present findings suggest that p40
may play a role in the regulation of NK and T lymphocyte activation and
proliferation.
ARTICLES
p40 molecule regulates NK cell activation mediated by NK receptors for HLA class I antigens and TCR-mediated triggering of T lymphocytes
Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Meyaard The inhibitory collagen receptor LAIR-1 (CD305) J. Leukoc. Biol., April 1, 2008; 83(4): 799 - 803. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Prevosto, M. Zancolli, P. Canevali, M. R. Zocchi, and A. Poggi Generation of CD4+ or CD8+ regulatory T cells upon mesenchymal stem cell-lymphocyte interaction Haematologica, July 1, 2007; 92(7): 881 - 888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Verbrugge, T. de Ruiter, C. Geest, P. J. Coffer, and L. Meyaard Differential expression of leukocyte-associated Ig-like receptor-1 during neutrophil differentiation and activation J. Leukoc. Biol., April 1, 2006; 79(4): 828 - 836. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Poggi, C. Prevosto, A.-M. Massaro, S. Negrini, S. Urbani, I. Pierri, R. Saccardi, M. Gobbi, and M. R. Zocchi Interaction between Human NK Cells and Bone Marrow Stromal Cells Induces NK Cell Triggering: Role of NKp30 and NKG2D Receptors J. Immunol., November 15, 2005; 175(10): 6352 - 6360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Poggi, S. Negrini, M. R. Zocchi, A.-M. Massaro, L. Garbarino, S. Lastraioli, L. Gargiulo, L. Luzzatto, and R. Notaro Patients with paroxysmal nocturnal hemoglobinuria have a high frequency of peripheral-blood T cells expressing activating isoforms of inhibiting superfamily receptors Blood, October 1, 2005; 106(7): 2399 - 2408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Poggi, A.-M. Massaro, S. Negrini, P. Contini, and M. R. Zocchi Tumor-Induced Apoptosis of Human IL-2-Activated NK Cells: Role of Natural Cytotoxicity Receptors J. Immunol., March 1, 2005; 174(5): 2653 - 2660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Verbrugge, T. d. Ruiter, H. Clevers, and L. Meyaard Differential contribution of the immunoreceptor tyrosine-based inhibitory motifs of human leukocyte-associated Ig-like receptor-1 to inhibitory function and phosphatase recruitment Int. Immunol., November 1, 2003; 15(11): 1349 - 1358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Spaggiari, P. Contini, A. Dondero, R. Carosio, F. Puppo, F. Indiveri, M. R. Zocchi, and A. Poggi Soluble HLA class I induces NK cell apoptosis upon the engagement of killer-activating HLA class I receptors through FasL-Fas interaction Blood, December 1, 2002; 100(12): 4098 - 4107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. I. Roberts, L. Lee, E. Schwarz, V. Groh, T. Spies, E. C. Ebert, and B. Jabri Cutting Edge: NKG2D Receptors Induced by IL-15 Costimulate CD28-Negative Effector CTL in the Tissue Microenvironment J. Immunol., November 15, 2001; 167(10): 5527 - 5530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lunardi, L. Nanni, M. Tiso, M. C. Mingari, C. Bason, M. Oliveri, B. Keller, R. Millo, G. De Sandre, R. Corrocher, et al. Glycine-rich cell wall proteins act as specific antigen targets in autoimmune and food allergic disorders Int. Immunol., May 1, 2000; 12(5): 647 - 657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Meyaard, J. Hurenkamp, H. Clevers, L. L. Lanier, and J. H. Phillips Leukocyte-Associated Ig-Like Receptor-1 Functions as an Inhibitory Receptor on Cytotoxic T Cells J. Immunol., May 15, 1999; 162(10): 5800 - 5804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Mandelboim, S. Kent, D. M. Davis, S. B. Wilson, T. Okazaki, R. Jackson, D. Hafler, and J. L. Strominger Natural killer activating receptors trigger interferon gamma secretion from T cells and natural killer cells PNAS, March 31, 1998; 95(7): 3798 - 3803. [Abstract] [Full Text] [PDF]
|
|