International Immunology, Vol 9, 815-823, Copyright © 1997 by Oxford University Press
PA Robbins, PA Rota and SZ Shapiro
The HLA restriction and epitope specificity of cytotoxic T lymphocytes
(CTL) involved in recovery from influenza type B infection have not been
extensively characterized. Here lymphocytes obtained from a healthy
individual contained virus-specific CTL restricted by class I HLA
molecules, HLA-A1, A2, B7 and B8, and the class II HLA molecules, HLA-DR1
and DR3. Four conserved viral epitopes were predicted from allele-specific
motifs for peptides interacting with HLA-B8 and HLA- DR1. Bulk CTL
recognized three 9mer HLA-B8-restricted peptides from nucleoprotein,
residues 30-38, 263-271 and 413-421, and a 13mer HLA-DR1- restricted
peptide from hemagglutinin, residues 308-320. The epitopes presented by
HLA-A1, HLA-B7 and HLA-DR3 remain undefined. Peptide- specific CTL lines
recognized influenza type B virus-infected cells indicating the peptides
are representative of naturally processed epitopes. A hemagglutinin
peptide-specific CD4 CTL clone expressed approximately 200 molecules of
perforin mRNA/cell, suggestive of a functional perforin pathway for target
cell lysis. The results indicate a broad CTL response composed of both CD8
CTL and CD4 CTL recognizing viral epitopes presented by multiple HLA
molecules.
ARTICLES
A broad cytotoxic T lymphocyte response to influenza type B virus presented by multiple HLA molecules
Center for Biologics Evaluation and Research, National Institutes of Health, Bethesda, MD 20892, USA.
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