International Immunology, Vol 9, 281-290, Copyright © 1997 by Oxford University Press
MH Wauben, M van der Kraan, MC Grosfeld-Stulemeyer and I Joosten
The Lewis rat, an inbred rat strain susceptible to several well-
characterized experimental autoimmune diseases, provides a good model to
study peptide-mediated immunotherapy. Peptide immunotherapy focussing on
the modulation of T cell responses by interfering with TCR- peptide-MHC
complex formation requires the elucidation of the molecular basis of
TCR-peptide-MHC interactions for an efficient design of modulatory
peptides. In the Lewis rat most autoimmune-associated CD4+ T cell responses
are MHC class II RT1.BL restricted. In this study, the characteristics of
RT1.BL-peptide interactions were explored. A series of substitution analogs
of two Lewis rat T cell epitopes was examined in a direct peptide-MHC
binding assay on isolated RT1.BL molecules. Furthermore, other
autoimmune-related as well as non-disease-related T cell epitopes were
tested in the binding assay. This has led to the definition of an extended
RT1.BL-peptide binding motif. The RT1.BL- peptide binding motif established
in this study is the first described rat MHC-peptide binding motif based on
direct MHC-peptide binding experiments. To predict good or intermediate
RT1.BL binding peptides, T cell epitope search profiles were deduced from
this motif. The motif and search profiles will greatly facilitate the
prediction of modulatory peptides based on autoimmune-associated T cell
epitopes and the identification of target structures in experimental
autoimmune diseases in Lewis rats.
ARTICLES
Definition of an extended MHC class II-peptide binding motif for the autoimmune disease-associated Lewis rat RT1.BL molecule
Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
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