International Immunology, Vol 9, 239-248, Copyright © 1997 by Oxford University Press
S Cho and DH Conrad
CHO cells permanently transfected with mouse Fc gamma RI alpha chain were
prepared and used as a model to polyclonally activate murine B cells. The
transfected CHO cells were treated with mitomycin C and placed into culture
with varying quantities of anti-IgD. Using this model, murine splenic B
cells (from BALB/c or C57Bl/6) were activated by mouse IgG2a-anti-IgD
(10.4.22 or AF3.33) in a manner that is analogous to the activation of B
cells seen with highly polyvalent anti- IgD (H delta(a)/1) prepared by
chemical cross-linking to dextran. Efficient B cell activation was seen
with nanogram quantities of anti- IgD. In the presence of IL-4 and IL-5,
IgG1 production levels were equivalent to or better than seen when
stimulation was with H delta(a)/1-dextran; however, IgE induction was not
seen in either situation. The Ig production capacity was compared to that
seen when B cells were activated with CD40L, using either CD40L-transfected
CHO or a soluble CD40L construct. In the presence of IL-4 and IL-5, once a
critical threshold of B cells was present, IgE and to a lesser extent IgG1
production was inversely proportional to B cell number when CD40L was the
activating agent. In contrast, with Fc gamma RI-anti-IgD, IgM and IgG1
production was directly proportional to B cell number, while IgE production
was never seen. Finally, when B cells were co-activated with immobilized
anti-IgD and CD40L simultaneously, the IgE production from B cells induced
by CD40L was strongly inhibited, while IgG1 and IgM production were not
affected. Since B cell co-activation via sIg and CD40L would be a common
scenario in secondary follicles, this inhibition of IgE production may be
one of the reasons why serum IgE levels are much below IgG in normal immune
situations.
ARTICLES
A new multivalent B cell activation model--anti-IgD bound to Fc gamma RI: properties and comparison with CD40L-mediated activation
Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298, USA.
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