International Immunology, Vol 9, 1849-1855, Copyright © 1997 by Oxford University Press
T Katakai, Y Agata, A Shimizu, C Ohshima, A Nishio, M Inaba, S Kasakura, KJ Mori and T Masuda
A parietal cell-specific Th1 clone, II-6, which was established from a
BALB/c mouse bearing post-thymectomy autoimmune gastritis (AIG), recognizes
a peptide of the alpha subunit (alpha891-905) of H+/K+- ATPase and induces
gastritis in nu/nu BALB/c mice by adoptive cell transfer. In the present
study, the primary structure of the TCR of II- 6 was determined as
Valpha10-Jalpha c5a-Calpha and Vbeta14-Jbeta2.3- Cbeta2 by cDNA cloning.
Using PCR with specific primers, we defined the use of this II-6 TCR in
nu/nu mice with transferred II-6 cells and in mice that spontaneously
developed AIG by thymectomy on day 3 after birth (d3-Tx). II-6 TCR mRNAs
were detected in the gastric mucosa of all of the nu/nu mice, suggesting
that II-6 cells indeed home to the gastric mucosa and thereby were directly
involved in the destruction of target parietal cells. TCR beta chain mRNAs
encoding CDR3 region sequences almost identical with that of II-6 were also
found in the gastric mucosa in 43% (six of 14 mice tested) of the d3-Tx AIG
mice at 4-12 weeks old by nested RT-PCR. Such a frequent appearance of
similar clonotypes in independent individuals suggests that T cells bearing
II- 6-like TCR including the II-6 itself might be directly involved in,
although not essential for, the pathogenesis of AIG in 3d-Tx mice.
ARTICLES
Structure of the TCR expressed on a gastritogenic T cell clone, II-6, and frequent appearance of similar clonotypes in mice bearing autoimmune gastritis
Institute for Immunology, Faculty of Medicine, Kyoto University, Japan.
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