International Immunology, Vol 9, 1475-1480, Copyright © 1997 by Oxford University Press
S Koyasu, LK Clayton, A Lerner, H Heiken, A Parkes and EL Reinherz
A rearranged TCR alpha transgene remains transcriptionally inactive in
rag-2-/- thymocytes but can be induced by CD3-mediated signals with
concomitant maturation of double-negative (DN) thymocytes to the CD4+CD8+
double-positive (DP) stage. Reciprocally, the same signals silence pre-TCR
alpha (pT alpha) expression. In normal C57BL/6 thymocytes, TCR alpha
expression is not detected in DN thymocytes while, in contrast, TCR beta
expression is initiated at the most immature c-kit+CD44+CD25- stage and
continues throughout thymocyte development. pT alpha expression is first
detected at the intermediate c-kit +/- CD44+CD25+ DN stage, increases
during transition to the more mature c-kit-CD44-CD25+ stage and is lost at
the DP stage. Thus, although TCR beta and pT alpha expression are
independent, the pre-TCR complex mediates signals controlling the
appearance of alpha beta TCR through selective regulation of TCR alpha and
pT alpha genes.
ARTICLES
Pre-TCR signaling components trigger transcriptional activation of a rearranged TCR alpha gene locus and silencing of the pre-TCR alpha locus: implications for intrathymic differentiation
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Xu, A. Sharma, D. L. Wiest, and J. M. Sen Pre-TCR-Induced {beta}-Catenin Facilitates Traversal through {beta}-Selection J. Immunol., January 15, 2009; 182(2): 751 - 758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Inoue, T. Kanefuji, K. Okazuka, H. Watanabe, Y. Mishima, and R. Kominami Expression of TCR{alpha}beta Partly Rescues Developmental Arrest and Apoptosis of {alpha}beta T cells in Bcl11b-/- Mice J. Immunol., May 15, 2006; 176(10): 5871 - 5879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Lefebvre, M. C. Haks, M. O. Carleton, M. Rhodes, G. Sinnathamby, M. C. Simon, L. C. Eisenlohr, L. A. Garrett-Sinha, and D. L. Wiest Enforced Expression of Spi-B Reverses T Lineage Commitment and Blocks {beta}-Selection J. Immunol., May 15, 2005; 174(10): 6184 - 6194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Grady, S. M. Mason, J. Stephen, J. C. Zuniga-Pflucker, and A. M. Michie Cyclic Adenosine 5'-Monophosphate Response Element Binding Protein Plays a Central Role in Mediating Proliferation and Differentiation Downstream of the Pre-TCR Complex in Developing Thymocytes J. Immunol., August 1, 2004; 173(3): 1802 - 1810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Carleton, M. C. Haks, S. A. A. Smeele, A. Jones, S. M. Belkowski, M. A. Berger, P. Linsley, A. M. Kruisbeek, and D. L. Wiest Early Growth Response Transcription Factors Are Required for Development of CD4-CD8- Thymocytes to the CD4+CD8+ Stage J. Immunol., February 15, 2002; 168(4): 1649 - 1658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Lacorazza, H. E. Porritt, and J. Nikolich-Zugich Dysregulated Expression of Pre-T{alpha} Reveals the Opposite Effects of Pre-TCR at Successive Stages of T Cell Development J. Immunol., November 15, 2001; 167(10): 5689 - 5696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Ramiro, M. N. Navarro, A. Carreira, Y. R. Carrasco, V. G. de Yebenes, G. Carrillo, J. L. San Millan, B. Rubin, and M. L. Toribio Differential Developmental Regulation and Functional Effects on Pre-TCR Surface Expression of Human pT{alpha}a and pT{alpha}b Spliced Isoforms J. Immunol., November 1, 2001; 167(9): 5106 - 5114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Lacorazza, C. Tucek-Szabo, L. V. Vasovic, K. Remus, and J. Nikolich-Zugich Premature TCR{{alpha}}{{beta}} Expression and Signaling in Early Thymocytes Impair Thymocyte Expansion and Partially Block Their Development J. Immunol., March 1, 2001; 166(5): 3184 - 3193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sasada and E. L. Reinherz A Critical Role for CD2 in Both Thymic Selection Events and Mature T Cell Function J. Immunol., February 15, 2001; 166(4): 2394 - 2403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Michie, S. Trop, D. L. Wiest, and J. C. Zuniga-Pflucker Extracellular Signal-Regulated Kinase (Erk) Activation by the Pre-T Cell Receptor in Developing Thymocytes in Vivo J. Exp. Med., December 6, 1999; 190(11): 1647 - 1656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Kim, X.-C. Peng, and X.-H. Sun Massive Apoptosis of Thymocytes in T-Cell-Deficient Id1 Transgenic Mice Mol. Cell. Biol., December 1, 1999; 19(12): 8240 - 8253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Carleton, N. R. Ruetsch, M. A. Berger, M. Rhodes, S. Kaptik, and D. L. Wiest Signals Transduced by CD3{epsilon}, But Not by Surface Pre-TCR Complexes, Are Able to Induce Maturation of an Early Thymic Lymphoma In Vitro J. Immunol., September 1, 1999; 163(5): 2576 - 2585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ghendler, A. Smolyar, H.-C. Chang, and E. L. Reinherz One of the CD3{varepsilon} Subunits within a T Cell Receptor Complex Lies in Close Proximity to the C{beta} FG Loop J. Exp. Med., May 4, 1998; 187(9): 1529 - 1536. [Abstract] [Full Text] [PDF]
|
|