International Immunology, Vol 9, 91-103, Copyright © 1997 by Oxford University Press
C Sedlik, E Deriaud and C Leclerc
Several factors are involved in the selective activation of Th1 or Th2
subset of CD4+ T cells, such as the type of antigen-presenting cells, the
dose of antigen, the route of immunization, etc. To analyze the influence
of accessory cells on Th1/Th2 cell differentiation, we used a particulate
antigen prepared by covalent linkage of hemocyanin (LH) to 1 microns
synthetic microspheres. This particulate antigen was efficiently presented
to T cells by macrophages but not by B lymphocytes. BALB/c mice immunized
either with soluble LH in alum or with particulate LH without adjuvant
produced both Th1 (IL-2 and IFN- gamma) and Th2 (IL-4 and IL-5) cytokines.
Moreover, mice primed either with soluble or particulate LH secreted higher
levels of IgG1- than of IgG2a-specific antibodies. The induction of this
cytokine profile response was independent of the route of administration of
the antigen, and was observed both in BALB/c and C57BL/6 mice. In contrast,
immunization of mice with particulate LH in the presence of poly(I):(C) or
of IL-12 induced a strong activation of Th1 cells, as shown by an up-
regulated IFN-gamma production, and by decreased IL-4 and IL-5 levels
associated to a greatly enhanced IgG2a antibody response. These results
therefore demonstrate that targeting the antigen to phagocytic cells is not
sufficient to stimulate a polarized Th response and that environmental
cytokines play the major role in the selective activation of Th1 cells.
This study provides important conclusions for the development of new
vaccines and shows that particulate antigen associated with appropriate
cofactor can selectively activate Th1 cells.
ARTICLES
Lack of Th1 or Th2 polarization of CD4+ T cell response induced by particulate antigen targeted to phagocytic cells
Unite de Biologie ces Regulations Immunitaires, Institut Pasteur, Paris, France.
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