International Immunology, Vol 9, 9-15, Copyright © 1997 by Oxford University Press
JC Guery, F Ria, F Galbiati, S Smiroldo and L Adorini
We have compared the capacity of dendritic cells (DC) and B cells to
present peptide-class II complexes following administration of protein in
adjuvant or in soluble form. Three different antigen-presenting cell (APC)
populations were separated from draining lymph node cells from mice
immunized s.c. with hen egg-white lysozyme (HEL) in adjuvant or with
adjuvant only followed by soluble HEL: DC (N418+, class II+, B220- , low
buoyant density), large B cells (B220+, low buoyant density) and small B
cells (B220+, high buoyant density). HEL peptide-class II complexes
displayed by these APC were evaluated by their capacity to activate
HEL-specific T hybridoma cells. Following immunization with HEL in
adjuvant, DC are the only lymph node APC population expressing detectable
HEL peptide-class II complexes. Conversely, after i.v. administration of
soluble HEL in mice previously injected with adjuvant only, lymph node B
cells are much more efficient than DC in presenting peptide-class II
complexes to T cells. Therefore, different modes of protein antigen
administration lead to selective expression of antigenic complexes by
different APC populations. These data correlate with the observation that,
unlike B cells, DC recruited in lymph nodes of mice injected with adjuvant
only present in vitro processed protein antigen much less efficiently than
synthetic peptides, probably as a consequence of their maturation in vivo.
ARTICLES
The mode of protein antigen administration determines preferential presentation of peptide-class II complexes by lymph node dendritic or B cells
Roche Milano Ficerche, Milano, Italy.
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