International Immunology, Vol 9, 27-41, Copyright © 1997 by Oxford University Press
X Chen, F Martin, KA Forbush, RM Perlmutter and JF Kearney
Antibody reactivity to self-antigens is a normal component of the immune
system. To study the mechanism by which self-reactive B cells are generated
and maintained, we analyzed B cell development in transgenic mice that
express a rearranged VH81X heavy chain from the pre-immune repertoire. In
these mice, > 95% of B cells express the transgene in association with a
variety of kappa light chains but V kappa 1 C being the dominant light
chain. These transgenic B cells with identical V kappa 1C-J kappa 5 joins
do not normally secrete IgM in vivo, but antibodies derived from these B
cells, through LPS activation in vitro or after hybridoma immortalization,
are self-reactive and recognize an ubiquitous epitope(s) on
intracytoplasmic proteins from different tissues. They have the phenotype
and localization pattern of long-lived marginal zone B cells and their
development in vivo is blocked by injection of soluble VH81X-V kappa 1CJ
kappa 5 IgM antibody. The observations in this transgenic mouse provide
evidence for positive selection of a population of self-reactive B cells.
These B cells enter the peripheral pool of B cells where they localize in
the marginal zone of the spleen and, in contrast to other
transgene-expressing B cells, do not secrete IgM antibody.
ARTICLES
Evidence for selection of a population of multi-reactive B cells into the splenic marginal zone
Department of Microbiology, University of Alabama at Birmingham 35294- 3300, USA.
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