International Immunology, Vol 9, 189-199, Copyright © 1997 by Oxford University Press
SJ Berens, DE Wylie and OJ Lopez
We have analyzed transcripts encoding the variable regions of Ig heavy
chains from adult and fetal bovine splenocytes and bovine x mouse
heterohybridomas. The 13 adult, seven fetal and two heterohybridomas
transcripts as well as the six genes that were sequenced had > 83%
identity to each other in the VH-encoded regions (FRs 1-3 and CDRs 1 and
2). By this criterion, all the bovine sequences were assigned to one
family, which corresponds to the bovine homolog of the murine Q52 family.
Southern blot analysis of genomic DNA demonstrated that homologs of other
murine VH families such as 7183, S107 and 36-60 were present in the genome,
but transcripts from these families were not detected in rapid
amplification of cDNA ends (RACE)-PCR amplified products or in individual
clones. The sequences of the adult transcripts using the mu isotype showed
extensive somatic mutation indicating that the process of somatic
hypermutation begins earlier in development of the bovine B cell. The
length of CDR3 from V(D)J rearrangements averaged 21 amino acids, which is
larger than other mammalian CDR3s. Analysis of CDR3s from 23 fetal
transcripts revealed a preference for a reading frame in the putative D
genes which is rich in glycine and tyrosine, and is also extensively
mutated in adults. The bovine immune system appears to utilize Ig VH genes
of a single family, but generates antibody diversity by extensive somatic
mutation and long CDR3s which are subsequently hypermutated.
ARTICLES
Use of a single VH family and long CDR3s in the variable region of cattle Ig heavy chains
School of Biological Sciences, University of Nebraska, Lincoln 68588- 0118, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Hosseini, G. Campbell, M. Prorocic, and R. Aitken Duplicated copies of the bovine JH locus contribute to the Ig repertoire Int. Immunol., June 1, 2004; 16(6): 843 - 852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhao, I. Kacskovics, H. Rabbani, and L. Hammarstrom Physical Mapping of the Bovine Immunoglobulin Heavy Chain Constant Region Gene Locus J. Biol. Chem., September 12, 2003; 278(37): 35024 - 35032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Saini, W. Farrugia, P. A. Ramsland, and A. K. Kaushik Bovine IgM antibodies with exceptionally long complementarity-determining region 3 of the heavy chain share unique structural properties conferring restricted VH + V{lambda} pairings Int. Immunol., July 1, 2003; 15(7): 845 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Johansson, M. Aveskogh, B. Munday, and L. Hellman Heavy Chain V Region Diversity in the Duck-Billed Platypus (Ornithorhynchus anatinus): Long and Highly Variable Complementarity-Determining Region 3 Compensates for Limited Germline Diversity J. Immunol., May 15, 2002; 168(10): 5155 - 5162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Charlton, S. Moyle, A. J. R. Porter, and W. J. Harris Analysis of the Diversity of a Sheep Antibody Repertoire as Revealed from a Bacteriophage Display Library J. Immunol., June 15, 2000; 164(12): 6221 - 6229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. O'Brien, R. Aitken, B. W. O'Neil, and M. S. Campo Generation of native bovine mAbs by phage display PNAS, January 19, 1999; 96(2): 640 - 645. [Abstract] [Full Text] [PDF]
|
|