International Immunology, Vol. 8, No. 9, pp. 1421-1428,September 1996
© 1996 Japanese Society for Immunology
Developmental control of antigen-induced thymic transcription factors
Centre d'immunologie INSERM-CNRS de Marseille Luminy, Parc Scientifique de Luminy Case 906, 13288 Marseille, France
Correspondence to: Correspondence to: A. K. Simon
Antigen engagement of the TCR may lead to activation of mature T cells while inducing deletion or positive selection of immature thymocytes. Using thymocytes from TCR transgenic mice recognizing the allo-antigen H-2Kb we investigated whether double-positive CD4+ CD8+ (DP) thymocytes constitute a particular developmental stage where signals originating from surface receptor engagement will lead to distinct nuclear signaling. We show that the developmental control of transcription factors is apparent, at least at two levels. First, NF-AT binding activity was not induced in response to either antigen or phorbol myristate acetate (PMA)/ionomycin in DP thymocytes, whereas it was induced in single-positive CD8 thymocytes. Second, antigen induced a different pattern of transcription factor binding activities than PMA/lonomycin in DP thymocytes, AP-1 activity being selectively induced by antigen and NF-kB by PMA/lonomycin. Further we show that the transcription factors found to be induced in the DP thymic population were not susceptible to the inhibitory effect of cyclosporin A.
Keywords: AP-1, NF-kB, NF-AT, thymic selection
Received 12 April 1996, accepted 6 June 1996.
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