International Immunology, Vol. 8, No. 1, pp. 91-99,January 1996
© 1996 Japanese Society for Immunology
Re-evaluation of the probabilities for productive rearrangements on the 
and
loci
1 Basel Institute for Immunology Grenzacherstrasse 487, 4005, Basel, Switzerland
Correspondence to: Correspondence to S. Takeda
V-J rearrangements at Ig light chain (IgL) genes occur in resting small pre-B cells. In the absence of cell division, the probability of productive 
and 
rearrangements is proportional to the output of + B and + B cells in bone marrow. The kinetics and probability of productive or rearrangements was assessed in three groups of mice carrying two (wild-type), one or no intact Ig gene, and the following conclusion are drawn, and rearrangements occur independently at different kinetics, and rearrangements are initiated at a time when rearrangements are stopping. The probability of productive and rearrangements per chromosome is calculated to be –60 and –20% respectively. Thus, a gene can attempt rearrangements up to three times per chromosome during B cell development. These findings explain that the observed ratio of + B/+ B cell production in wild-type mice is 95/5.
Keywords: allelic exclusion, B cell development, Ig gene rearrangement, lg, isotypic exclusion, RS recombination, V-J recombination
2Present address: Department of Biophysics, Faculty of Science, Kyoto University, Kyoto 606-01, Japan
3Department of Molecular immunology, Faculty of Medicine, Kyoto University, Kyoto 606-01, Japan
Received 7 August 1995, accepted 20 September 1995.
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