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International Immunology, Vol. 7, No. 3, pp. 493-499,March 1995
© 1995 Japanese Society for Immunology

TCR repertoire in early fetal mouse thymus

Hiroko Ohki-Hamazaki1,, Yasuhiko Makino, Masamoto Kanno, Haruhiko Koseki, Takeshi Akasaka and Masaru Taniguchi

Division of Molecular Immunology, Center for Biomedical Science, School of Medicine, Chiba University Chiba 260, Japan
1Present address. Division of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry Tokyo 187, Japan

Correspondence to: Correspondence to: H Ohki-Hamazaki

We investigated the rearrangement and expression of TCR genes in mouse fetal thymus organ culture, a system that avoids subsequent entry of hematopoietic precursor cells. The first observable rearranged TCR gene was homogeneous V{gamma}2-J{gamma}2, detectable as early as fetal day 11 (d11) in the thymic primordla. The productive TCR was homogeneous V{gamma}5-J{gamma}1, first detectable in d13 thymocytes, followed by adult-type TCR {gamma} (V{gamma}4 and V{gamma}7). Sequence analysis of TCR revealed five types of V-J junctional sequences. In the very early stage, a homogeneous V-J junction is generated via a short homology sequence in the coding region (Type I), while a short homology sequence in the P-nucleotlde rather than the coding region is used in the following stage (Type II). In the later embryonic stages, diverse V-J junctions are generated by well-known mechanisms, such as P-nucleotide (Type III), N-region insertion (Type IV) or trimming of the coding ends (Type V). These findings suggest that the generation of homogeneous TCR {gamma} (V{gamma}2 and V{gamma}5) in the early fetal stages is due to the intrinsic rearrangement mechanisms and is in stage specific manner.

Keywords: fetal thymus, P-nucleotide, short homology sequence, TCR rearrangements, TCR repertoire, V-J junctional sequence

Received 26 September 1994, accepted 21 November 1994.


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