International Immunology, Vol. 7, No. 3, pp. 471-479,March 1995
© 1995 Japanese Society for Immunology
Human vascular endothelial cells process and present autoantigen to human T cell lines
Center for Clinical Research in Immunology and Signalling, The Medical School, University of Birmingham Birmingham B15 2TT, UK
1 Neurosciences Group, Institute for Molecular Medicine, John Radcliffe Hospital Oxford OX3 9DU, UK
2 Transplantation Biology Section, Clinical Research Centre Harrow HA1 3UJ, UK
3 Bath Institute for Rheumatic Diseases Bath, Avon BA1 1HD, UK
Correspondence to: Correspondence to: C. O. S. Savage
The effectiveness of cultured human umbilical vein endothelial cells as accessory cells for T cell activation has been investigated using T cell clones and lines derived from patients with myasthenla gravis which were specific for different epitopes on the 
subunit of the human acetylcholine receptor. The endothelial cells were induced with IFN-
to express HLA-DR and -DQ at high and low levels respectively. They could then efficiently present specific peptides of the subunit to an HLA-DR- and an HLA-DQw5-restricted T cell line. They could also process epitopes for both T cell lines from the full-length recombinant a subunlt (r1—437) of the human acetylcholine receptor, where the known epitopes are 80 amino acid residues apart. The endothelial presentation of r1—437, but not of the peptides, was sensitive to chloroquine inhibition. Presentation appeared slightly less efficient (by 1.5- to 3.0-fold) with endothelial cells than with presenting cells from peripheral blood. This may reflect differences in accessory signalling since mAb blocking studies suggested that ligands for CD28 provided important accessory signalling by peripheral blood presenting cells while LFA-3 was used by endothelial cells.
Keywords: antigen presentation, endothelial cells, T lymphocytes
Received 9 June 1994, accepted 29 November 1994.
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