Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Aebischer, T.
Right arrow Articles by Handman, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aebischer, T.
Right arrow Articles by Handman, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 6, No. 10, pp. 1535-1543,October 1994
© 1994 Japanese Society for Immunology

Intravenous injection of irradiated Leishmania major into susceptible BALB/c mice: immunization or protective tolerance

Toni Aebischer, Lynn Morris and Emanuela Handman

The Walter and Eliza Hall Institute of Medical Research PO Royal Melbourne Hospital, Victoria 3050, Australia

Correspondence to: Correspondence to. E. Handman

It is well established that BALB/c mice can be protected from fatal infection with Leishmania major by prophylactic intravenous (i.v.) immunization with irradiated parasites. Protection is critically dependent on the route of injection with i.v. injection being protective and subcutaneous injection not protective. We used this BALB/c-L major model system to investigate this phenomenon. We analyzed quantitatively the parasite-specific, CD4+ T cell mediated immune responses by limiting dilution. Subcutaneous vaccination resulted in priming of CD4+ precursor T cells, whereas i.v. vaccination was ineffectual. Moreover, i.v. injection prevented the increase in the number of specific precursor cells induced by infection of normal mice during the first weeks post-challenge with virulent parasites. We show here that this was not due to the elimination of the virulent challenge parasites as a result of immunity nor to inefficient antigen presentation of the irradiated organisms after i.v. injection, The data presented here suggest that i.v. injection results in tolerizatlon rather than immunization. Tolertzation as a mechanism of host protection is consistent with earlier observations that transient immunosuppresslon results in cure of L. major infection in BALB/c mice. Transfer of antigen presenting cells (APC) isolated from spleens of mice injected previously with irradiated parasites mimicked to some extent the effect of i.v. immunization with irradiated parasites. The possible involvement of these APC in decreasing the parasite-specific T cell response is discussed.

Keywords: intravenous immunization, limiting dilution analysis, lymphokines, T cell subsets

Received 22 April 1994, accepted 23 June 1994.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
T. Aebischer, M. Wolfram, S. I. Patzer, T. Ilg, M. Wiese, and P. Overath
Subunit Vaccination of Mice against New World Cutaneous Leishmaniasis: Comparison of Three Proteins Expressed in Amastigotes and Six Adjuvants
Infect. Immun., March 1, 2000; 68(3): 1328 - 1336.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. J. Stacey and J. M. Blackwell
Immunostimulatory DNA as an Adjuvant in Vaccination against Leishmania major
Infect. Immun., August 1, 1999; 67(8): 3719 - 3726.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. R. Gabaglia, B. Pedersen, M. Hitt, N. Burdin, E. E. Sercarz, F. L. Graham, J. Gauldie, and T. A. Braciak
A Single Intramuscular Injection with an Adenovirus- Expressing IL-12 Protects BALB/c Mice Against Leishmania major Infection, While Treatment with an IL-4-Expressing Vector Increases Disease Susceptibility in B10.D2 Mice
J. Immunol., January 15, 1999; 162(2): 753 - 760.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C Peters, T Aebischer, Y. Stierhof, M Fuchs, and P Overath
The role of macrophage receptors in adhesion and uptake of Leishmania mexicana amastigotes
J. Cell Sci., January 12, 1995; 108(12): 3715 - 3724.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.