International Immunology, Vol. 5, No. 9, pp. 1139-1150,September 1993
© 1993 Japanese Society for Immunology
CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection
Department of Immunology, Faculty of Medicine, University of Tokyo 7-3-1 Hongo Bunkyo-ku, Tokyo 113, Japan
Correspondence to: Correspondence to: T. Nakayama
CD69, an ‘activation marker’ that is rapidly induced on mature T cells after stimulation through the T cell antigen receptor (TCR) was found to be expressed on 
10% of normal thymocytes. All of these CD69+ thymocytes express 
ß TCR, and they include both TCRlowCD4+CD8+ and TCRhighCD4+CD8– or CD4–CD8+ thymocytes. The CD69+ cells can be further segregated into heat-stable antigen (HSA)+TCR*HSA–TCRhigh and HSA+TCRhigh thymocyte populations. None of CD69+ cells express the mature T cell marker Qa-2. Thus CD69+ cells present in vivo appear phenotyplcally to represent transitional cell populations between immature TCRlowHSA+Qa-2– double-positive cells and mature TCRhighHSA–QA-2+ single-positive cells. In addition, TCR engagement by MHC molecules is required for CD69 expression in the thymus. Taken together, the CD69 + thymocytes appear to represent the cells auditioning in positive selection process or they are the cells that have been positively selected recently. Analysis of a TCR transgenic mouse model revealed an increased number of CD69+ thymocytes in a positively selecting thymus, whereas no CD69+ transgenic TCR+ thymocytes were observed in the non-selecting thymus. Based on the results of this study, we suggest that the surface expression of CD69 serves as a useful marker to identify and trace those thymocytes that are engaged in the TCR-mediated positive selection process in the thymus.
Keywords: activation marker, CD4+CD8+ thymocytes, CD69, positive selection, T cell antigen receptor
Received 15 April 1993, accepted 8 June 1993.
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