Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Slieker, W. A. T.
Right arrow Articles by van Ewijk, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Slieker, W. A. T.
Right arrow Articles by van Ewijk, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 5, No. 9, pp. 1099-1107,September 1993
© 1993 Japanese Society for Immunology

ER-MP12 antigen, a new cell surface marker on mouse bone marrow cells with thymus-repopulating ability: II. Thymus-homing ability and phenotypic characterization of ER-MP12-positive bone marrow cells

Walentina A. T. Slieker1,, Johannes C. M. van der Loo2, Marella F. T. R. de Riik-de Bruijn1, Dale I. Godfrey3, Pieter J. M. Leenen1 and Willem van Ewijk1

1 Department of Immunology, Erasmus University 3000 DR Rotterdam, The Netherlands
2 Department of Hematology, Erasmus University 3000 DR Rotterdam, The Netherlands
3 DNAX Research Institute Palo Alto, CA, USA

Correspondence to: Correspondence to: W. A. T. Slieker

In the accompanying paper we showed that six distinct subsets of bone marrow (BM) cells can be identified using the mAb ER-MP12 and ER-MP20 in two-colour immunofluorescence analysis. Upon intrathymic transfer into sublethally irradiated mice thymus-repopulating ability was restricted to ER-MP20– BM cells expressing either high or intermediate levels of the ER-MP12 antigen (1–2% and –30% of BM nucleated cells respectively). The highest frequency of thymus-repopulating cells was found in the minor subset of ER-MP12++20– BM cells. In the present study we demonstrate that upon intravenous transfer, thymus-homing and-repopulating BM cells are exclusively confined to the ER-MP12++20– and ER-MP12+20– subpopulations, the highest frequency being detected among ER-MP12++20– BM cells. Analysis of the peripheral blood leucocytes of reconstituted mice showed that not only prothymocytes but also progenitorcells of the B cell lineage as well as the myelold lineage were present within both subsets. Three-colour flow cytometric analysis revealed that ER-MP12++20– BM cells in particular were phenotyplcally heterogeneous with respect to the expression of the cell surface markers Thy-1, Sca-1, CD44, B220 and c-kit. Taken together our data demonstrate that ER-MP12 positively identifies BM cells with the ability to home to and repopulate the thymus. The phenotypic heterogeneity displayed by the ER-MP12++20– BM subset, containing the highest frequency of thymus-homing and-repopulating cells, provides a basis for further separation of prothymocyte activity from other haematopoietic activities in the BM of the mouse.

Keywords: bone marrow subpopulation, ER-MP20, intravenous cell transfer, prethymic, prothymocyte, T cell development

Received 30 December 1992, accepted 28 May 1993.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Exp. Biol. Med.Home page
M. Jin, H. Jeon, H. J. Jung, B. Kim, S. S. Shin, J. J. Choi, J. K. Lee, C.-Y. Kang, and S. Kim
Enhancement of Repopulation and Hematopoiesis of Bone Marrow Cells in Irradiated Mice by Oral Administration of PG101, a Water-Soluble Extract from Lentinus lepideus
Experimental Biology and Medicine, June 1, 2003; 228(6): 759 - 766.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. S. van Rijt, J.-B. Prins, P. J. M. Leenen, K. Thielemans, V. C. de Vries, H. C. Hoogsteden, and B. N. Lambrecht
Allergen-induced accumulation of airway dendritic cells is supported by an increase in CD31hiLy-6Cneg bone marrow precursors in a mouse model of asthma
Blood, November 15, 2002; 100(10): 3663 - 3671.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
H. Tagoh, R. Himes, D. Clarke, P. J.M. Leenen, A. D. Riggs, D. Hume, and C. Bonifer
Transcription factor complex formation and chromatin fine structure alterations at the murine c-fms (CSF-1 receptor) locus during maturation of myeloid precursor cells
Genes & Dev., July 1, 2002; 16(13): 1721 - 1737.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. A. Cancelas, W. L. M. Koevoet, A. E. de Koning, A. E. M. Mayen, E. J. C. Rombouts, and R. E. Ploemacher
Connexin-43 gap junctions are involved in multiconnexin-expressing stromal support of hemopoietic progenitors and stem cells
Blood, July 15, 2000; 96(2): 498 - 505.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
N. Faust, F. Varas, L. M. Kelly, S. Heck, and T. Graf
Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages
Blood, July 15, 2000; 96(2): 719 - 726.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
U. Jagle, A. M. Muller, H. Kohler, and C. Bonifer
Role of Positive and Negative Cis-regulatory Elements in the Transcriptional Activation of the Lysozyme Locus in Developing Macrophages of Transgenic Mice
J. Biol. Chem., February 28, 1997; 272(9): 5871 - 5879.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.