Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (24)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Slieker, W. A. T.
Right arrow Articles by van Ewijk, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Slieker, W. A. T.
Right arrow Articles by van Ewijk, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 5, No. 9, pp. 1093-1098,September 1993
© 1993 Japanese Society for Immunology

ER-MP12 antigen, a new cell surface marker on mouse bone marrow cells with thymus-repopulating ability: I. Intrathymic repopulating ability of ER-MP12-positive bone marrow cells

Walentina A. T. Slieker, Marella F. T. R. de Rijk-de Bruijn, Pieter J. M. Leenen and Willem van Ewijk

Department of Immunology, Erasmus University 3000 DR Rotterdam, The Netherlands

Correspondence to: Correspondence to: W. A. T. Slieker

We searched for new cell surface markers that allow a positive identification of thymusrepopulating cells in the bone marrow (BM) of the mouse. Recently we raised two rat monocional antibodies (ER-MP12 and ER-MP20) that recognize cell surface antigens expressed by mouse haematopoietic progenitor cells, among which are progenitor cells of the macrophage lineage. Here we show that the ER-MP12 antigen, but not the ER-MP20 antigen, is also expressed by BM cells with thymus-repopulating ability. Using ER-MP12 and ER-MP20 in two-colour immunofluorescence analysis six subpopulatlons of BM cells can be identified. The thymusrepopulating ability of each BM subpopulation was assessed after fluorescence-activated cell sorting and subsequent intrathymic injection into sublethally irradiated Thy-1 congenic recipient mice. Thymus-repopulating activity appeared to be exclusively confined to two subsets of BM cells expressing either high or intermediate levels of the ER-MP12 antigen, but lacking ER-MP20 antigen expression. These BM subsets comprised 1–2% and 30% of total nucleated BM cells respectively. The frequency of thymus-repopulating cells was maximal in the minor BM subpopulation with the highest level of ER-MP12 antigen expression. We conclude that ER-MP12 detects a hitherto unknown cell surface marker expressed by BM cells with thymus-repopulating ability.

Keywords: bone marrow subpopulation, ER-MP20, intrathymic cell transfer, prethymic, prothymocyte, T cell development

Received 30 December 1992, accepted 28 May 1993.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
E. J. Kuiper, P. Roestenberg, C. Ehlken, V. Lambert, H. B. van Treslong-de Groot, K. M. Lyons, H.-J. T. Agostini, J.-M. Rakic, I. Klaassen, C. J.F. Van Noorden, et al.
Angiogenesis Is Not Impaired in Connective Tissue Growth Factor (CTGF) Knock-out Mice
J. Histochem. Cytochem., November 1, 2007; 55(11): 1139 - 1147.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
T. Nikolic, M. F. T. R. d. Bruijn, M. B. Lutz, and P. J. M. Leenen
Developmental stages of myeloid dendritic cells in mouse bone marrow
Int. Immunol., April 1, 2003; 15(4): 515 - 524.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G. W. Henkel, S. R. McKercher, P. J.M. Leenen, and R. A. Maki
Commitment to the Monocytic Lineage Occurs in the Absence of the Transcription Factor PU.1
Blood, May 1, 1999; 93(9): 2849 - 2858.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. Zhou, L. Qian, C. K. Bieszczad, R. Noelle, M. Binder, N. B. Levy, and R. W. Craig
Mcl-1 in Transgenic Mice Promotes Survival in a Spectrum of Hematopoietic Cell Types and Immortalization in the Myeloid Lineage
Blood, November 1, 1998; 92(9): 3226 - 3239.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.