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International Immunology, Vol. 5, No. 9, pp. 1041-1048,September 1993
© 1993 Japanese Society for Immunology

Synergistic interactions of CD4+ and CD8+ T cell subsets with human vascular endothelial cells in primary proliferative allogeneic responses

Christopher J. Brooks, Arthur Stackpoole and Caroline O. S. Savage

Vascular Biology Team, Section of Transplantation Biology, Clinical Research Centre Harrow HA1 3UJ, UK

Correspondence to: Correspondence to: C. O. S. Savage

The ability of subcultured human vascular endothelial cells (EC) to provide immune accessory functions for proliferative responses of highly purified allogeneic CD4+ and CD8+ T cells has been examined. CD4+ T cells proliferated in response to IFN-{gamma}-pretreated EC which expressed class II molecules, but not to untreated EC. CD8+ T cells proliferated to MHC class I molecules expressed on both untreated and IFN-{gamma}-treated EC. Combined populations of CD4+ and CD8+ T cells showed synergistic, rather than additive, responses to both untreated and IFN-{gamma}-treated EC. Furthermore, CD8+ T cells were able to induce MHC class II expression on endothelial cells and this induction could be inhibited by an anti-IFN-{gamma} mAb. The synergistic response obtained by co-culturing CD4+ and CD8+ T cells with vascular EC was completely inhibited by the sameantl-IFN-{gamma} mAb. These studies suggest that CD4+ and CD8+ T cells recognise and proliferate to allogeneic MHC molecules expressed by EC. CD4+ and CD8+responses are synergistic under the conditions tested and this synergism appears to be due to induction of MHC class II antigens on C by IFN-{gamma} secreted from CD8+ T cells.

Keywords: endothelial cells, interferon-{gamma}, major histocompatibility complex, peripheral blood lymphocytes

Received 12 January 1993, accepted 17 May 1993.


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