International Immunology, Vol. 5, No. 9, pp. 1035-1039,September 1993
© 1993 Japanese Society for Immunology
Effect of age on the expressed B cell repertoire: role of B cell subsets
Department of Medicine, Cornell University Medical College New York, NY 10021, USA
Correspondence to: Correspondence to: M. E. Weksler
Aged humans and experimental animals are impaired in their responses to most foreign antigens although they produce greater amounts of autoantibodies. We have examined the effect of age on the production of antibodies to a prototypic foreign antigen, sheep erythrocytes (SRBC), and to a prototypic autoantigen, bromelain-treated mouse erythrocytes (BrMRBC), in young and old ice before and after immunization with SRBC. Old mice express more anti-BrMRBC plaqueforming cell (PFC) antibodies before and an even greater number after immunization with SRBC than young mice. Conversely, old mice produce far fewer anti-SRBC PFC than young mice following immunization with SRBC. We hypothesized that the differences in the responses of old mice to BrMRBC and SRBC reflects differences in the activity of CD5+ and CD5– B cells. To test this hypothesis we immunized young and old mice with foreign antigens reported (and confirmed in our studies) to stimulate CD5+ B cells [TNP-ficoll and phosphorylcholine - keyhole limpet hemocyanln (KLH)] or CD5– B cells (SRBC and TNP-KLH). We found that the PFC response of old mice to antigens mediated by CD5+ B cells was equal to or greater than that of young mice. In contrast the PFC response of old mice induced by antigens mediated by CD5+ B cells was only 10% that of young mice. Thus it appears that the immune response of old mice is well maintained for antigens which elicit a CD5+ B cell response but not for those which elicit a CD5– B cell response. This conclusion is supported by our finding that a higher percentage of splenic B cells from old compared to young mice express that VH11 lgh gene family. This VH gene family is preferentially expressed by CD5+ B cells.
Keywords: CD5+ B cells, foreign antigens, immune senescence, mice
Received 4 January 1993, accepted 15 May 1993.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  G. L. Goldberg, C. G. King, R. A. Nejat, D. Y. Suh, O. M. Smith, J. C. Bretz, R. M. Samstein, J. A. Dudakov, A. P. Chidgey, S. Chen-Kiang, et al. Luteinizing Hormone-Releasing Hormone Enhances T Cell Recovery following Allogeneic Bone Marrow Transplantation J. Immunol., May 1, 2009; 182(9): 5846 - 5854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Simell, M. Lahdenkari, A. Reunanen, H. Kayhty, and M. Vakevainen Effects of Ageing and Gender on Naturally Acquired Antibodies to Pneumococcal Capsular Polysaccharides and Virulence-Associated Proteins Clin. Vaccine Immunol., September 1, 2008; 15(9): 1391 - 1397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Goldberg, O. Alpdogan, S. J. Muriglan, M. V. Hammett, M. K. Milton, J. M. Eng, V. M. Hubbard, A. Kochman, L. M. Willis, A. S. Greenberg, et al. Enhanced Immune Reconstitution by Sex Steroid Ablation following Allogeneic Hemopoietic Stem Cell Transplantation J. Immunol., June 1, 2007; 178(11): 7473 - 7484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Labrie III, A. P. Sah, D. M. Allman, M. P. Cancro, and R. M. Gerstein Bone Marrow Microenvironmental Changes Underlie Reduced RAG-mediated Recombination and B Cell Generation in Aged Mice J. Exp. Med., August 16, 2004; 200(4): 411 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Frasca, E. Van der Put, R. L. Riley, and B. B. Blomberg Reduced Ig Class Switch in Aged Mice Correlates with Decreased E47 and Activation-Induced Cytidine Deaminase J. Immunol., February 15, 2004; 172(4): 2155 - 2162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Jayashankar, K. M. Brasky, J. A. Ward, and R. Attanasio Lymphocyte Modulation in a Baboon Model of Immunosenescence Clin. Vaccine Immunol., September 1, 2003; 10(5): 870 - 875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Frasca, D. Nguyen, R. L. Riley, and B. B. Blomberg Decreased E12 and/or E47 Transcription Factor Activity in the Bone Marrow As Well As in the Spleen of Aged Mice J. Immunol., January 15, 2003; 170(2): 719 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Johnson, S. J. Rozzo, and J. C. Cambier Aging-Dependent Exclusion of Antigen-Inexperienced Cells from the Peripheral B Cell Repertoire J. Immunol., May 15, 2002; 168(10): 5014 - 5023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Anspach, G. Poulsen, I. Kaattari, R. Pollock, and P. Zwollo Reduction in DNA Binding Activity of the Transcription Factor Pax-5a in B Lymphocytes of Aged Mice J. Immunol., February 15, 2001; 166(4): 2617 - 2626. [Abstract] [Full Text] [PDF]
|
|