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International Immunology, Vol. 4, No. 11, pp. 1219-1223,November 1992
© 1992 Japanese Society for Immunology
Accumulation of multiple T cell clonotypes in the synovial lesions of patients with rheumatoid arthritis revealed by a novel clonality analysis
1 Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo 7-3-1, Hongo, Bunkyo-ku, Tokyo 113, Japan
2 Division of Rheumatology and Molecular Immunology, Institute of Medical Science, St Marianna University, School of Medicine 2-16-1, Sugao, Kawasaki 216, Japan
Correspondence to: Correspondence to: K. Yamamoto
T cell activation in the characteristic synovial lesions of rheumatoid arthritis may play a major role in the pathogenesis of this autoimmune disease. Analysis of T cell clonal diversity in these sites remains equivocal. Using the PCR and subsequent single-strand conformation polymorphism analysis it is possible to assess the degree of junctional diversity In the TCR with minimal selection bias. Concentrating on the ß-chain of the TCR, a paucity of clonotypic T cell expansion is demonstrated in the peripheral blood of healthy Individuals. After polyclonal stimulation in vitro (with concanavalln A or phytohemagglutlnln) this pattern does not change. In contrast, some T cell clonotypes appear following in vitro stimulation with purified protein derivative. Analysis of the peripheral blood, synovial fluid, and synovial tissue of patients with rheumatoid arthritis indicated many dominant T cell clonotypes. These data argue for a clonally diverse T cell response In the affected tissues of rheumatoid arthritic subjects.
Keywords: polymerase chain reaction, single-strand conformation polymorphism, T cell receptor
Received 29 May 1992, accepted 15 July 1992.
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