International Immunology, Vol. 4, No. 1, pp. 83-92,January 1992
© 1992 Japanese Society for Immunology
Identification of distinct epitopes of endoglin, an RGD-containing glycoprotein of endothelial cells, leukemic cells, and syncytiotrophoblasts
Division of Immunology and Cancer Research, The Hospital for Sick Children 555 University Avenue, Toronto, Canada M5G 1XB
1 Department of Clinical Immunology, Beth Israel Hospital Boston, MA 02215, USA
2 Department of Clinical Immunology, St Vincent's Hospital Fitzroy, 3065, Australia
3 Second Department of Internal Medicine, Medical University Clinic of Tübingen 7400 Tübingen, Germany
4 Center for Biological Investigation 28006 Madrid, Spain
5 The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service 1066 CX Amsterdam, The Netherlands
Correspondence to: Correspondence to: M. Letarte
Endoglin is a glycoprotein expressed predominantly on human endothelial cells. It was first identified with mAb 44G4, produced against the pre-B acute lymphoblastic HOON cell line. We now report that four mAbs Independently produced against human umbilical vein endothelial cells (HUVECs), chronic myelogenous leukemia in blast crisis, or U-937 pro-monocytic cells stimulated with phorbol myristate acetate also react with endoglin. High levels of reactivity of all mAbs were observed with HUVEC, while intermediate levels were seen with HOON and U-937 cells. By sequential Immunoprecipitation from HUVEC and U-937 cell extracts, it was established that RMAC8, HEC-19, 8E11, and 1G2 mAbs react with the same protein as 44G4. Three distinct epitopes recognized by 44G4, RMAC8, and 1G2 mAbs were identified by competitive radioimmunoassay and flow cytometry. The HEC-19 epitope is spatially related to the 44G4 epitope, whereas the 8E11 epitope Is most closely related to the 1G2 epitope. Western blot analysis showed that all antibodies react with the endoglin dimer (Mr = 170, 000) purified from placenta. Immunostaining of sections of full-term placenta revealed reactivity not only with fetal vessels but also with the syncytlotrophoblast, the fetal cell layer which interfaces with maternal blood. When HUVEC monolayers were treated with the different mAbs to endoglin, prior to Incubation with U-937 cells, a 5- to 10-fold stimulation of adhesion was observed. A fibronectin hexapeptide containing RGD, but not the corresponding RGE peptlde, was capable of Inhibiting the increased adhesion, when tested with mAb 44G4 and RMAC8. However, the same peptides had no effect on the binding of any of the five anti-endoglin mAbs to cells. Since 44G4 and RMAC8 recognize two distinct epitopes of endoglin, and since all five mAbs stimulated adhesion, the results suggest that a signal has been triggered through endoglin on HUVECs. Endoglin might be implicated either directly, by binding to a specific integrin-like ligand, or indirectly, by regulating the level of adhesion between certain integrins and their receptors.
Keywords: adhesion, placenta, Arg-Gly-Asp, monoclonal antibodies, myeloid antigen
Received 14 August 1991, accepted 10 October 1991.
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