International Immunology, Vol. 3, No. 4, pp. 343-351,April 1991
© 1991 Japanese Society for Immunology
The E1b oncogene of adenovirus confers cellular resistance to cytotoxicity of tumor necrosis factor and monoclonal anti-Fas antibody
Meiji Institute of Health Science 540 Naruda, Odawara 250, Japan
1 Department of Cell Biology, The Tokyo Metropolitan Institute of Medical Science Bunkyo-ku, Tokyo 113, Japan
Correspondence to: S. Hashimoto
The cell lines KB8, 16, and 18 are KB cells which constitutively express adenovirus type 2 (Ad2) E1a, E1a plus E1b, and E1b genes, respectively. We show here that KB18 cells are completely resistant to cytolysis by tumor necrosis factor (TNF) or anti-Fas, although KB8 and KB or KB16 are highly and moderately sensitive, respectively. The levels of receptors for TNF and anti-Fas of KB18 were almost the same as compared with those of KB or other KB-cell lines. Expression of manganous superoxide dismutase (MnSOD) mRNA in KB18 was about 20-fold higher than that in KB or KB8 cells. KB, HT29, and A673 cells infected with d/337 (an Ad5 mutant defective in E1b function) are highly sensitive to TNF or anti-Fas, although wild-type Ad2-infected cells are resistant. Our results indicate that the E1b oncogene can confer cellular resistance to cytolysls by either TNF or anti-Fas In both KB cells and adenovirus-infected human cell lines through influencing intracellular events including regulation of MnSOD genes. Furthermore, we describe how anti-Fas mimics only the cytolytlc activity of TNF, whereas TNF also has many other biological activities.
Keywords: adenovirus E1a gene, manganous superoxide, dismutase, fas-antigen, KB cells
Received 7 November 1990, accepted 8 January 1991.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Q. Li, A. K.-K. Ching, B. C.-L. Chan, S. K.-Y. Chow, P.-L. Lim, T. C.-Y. Ho, W.-K. Ip, C.-K. Wong, C. W.-K. Lam, K. K.-H. Lee, et al. A Death Receptor-associated Anti-apoptotic Protein, BRE, Inhibits Mitochondrial Apoptotic Pathway J. Biol. Chem., December 10, 2004; 279(50): 52106 - 52116. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cuconati and E. White Viral homologs of BCL-2: role of apoptosis in the regulation of virus infection Genes & Dev., October 1, 2002; 16(19): 2465 - 2478. [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lober, C. Lenz-Stoppler, and M. Dobbelstein Adenovirus E1-transformed cells grow despite the continuous presence of transcriptionally active p53 J. Gen. Virol., August 1, 2002; 83(8): 2047 - 2057. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. Tollefson, K. Toth, K. Doronin, M. Kuppuswamy, O. A. Doronina, D. L. Lichtenstein, T. W. Hermiston, C. A. Smith, and W. S. M. Wold Inhibition of TRAIL-Induced Apoptosis and Forced Internalization of TRAIL Receptor 1 by Adenovirus Proteins J. Virol., October 1, 2001; 75(19): 8875 - 8887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Shrivastava, S. K. Manna, R. Ray, and B. B. Aggarwal Ectopic Expression of Hepatitis C Virus Core Protein Differentially Regulates Nuclear Transcription Factors J. Virol., December 1, 1998; 72(12): 9722 - 9728. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Han, H. D. Wallen, G. Nuñez, and E. White E1B 19,000-Molecular-Weight Protein Interacts with and Inhibits CED-4-Dependent, FLICE-Mediated Apoptosis Mol. Cell. Biol., October 1, 1998; 18(10): 6052 - 6062. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Perez and E. White E1B 19K Inhibits Fas-mediated Apoptosis through FADD-dependent Sequestration of FLICE J. Cell Biol., June 1, 1998; 141(5): 1255 - 1266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Ray, K. Meyer, R. Steele, A. Shrivastava, B. B. Aggarwal, and R. Ray Inhibition of Tumor Necrosis Factor (TNF-alpha )-mediated Apoptosis by Hepatitis C Virus Core Protein J. Biol. Chem., January 23, 1998; 273(4): 2256 - 2259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. P. Limbourg, H. Stadtler, G. Chinnadurai, P. A. Baeuerle, and M. L. Schmitz A Hydrophobic Region within the Adenovirus E1B 19kDa Protein Is Necessary for the Transient Inhibition of NF-kappa B Activated by Different Stimuli J. Biol. Chem., August 23, 1996; 271(34): 20392 - 20398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Han, P Sabbatini, D Perez, L Rao, D Modha, and E White The E1B 19K protein blocks apoptosis by interacting with and inhibiting the p53-inducible and death-promoting Bax protein. Genes & Dev., February 15, 1996; 10(4): 461 - 477. [Abstract] [PDF]
|
|
|
|
|
|
E White Life, death, and the pursuit of apoptosis. Genes & Dev., January 1, 1996; 10(1): 1 - 15. [PDF]
|
|
|
|
 |
|
 B. Hay, T Wolff, and G. Rubin Expression of baculovirus P35 prevents cell death in Drosophila Development, January 8, 1994; 120(8): 2121 - 2129. [Abstract] [PDF]
|
|
|
|
 |
|
 E. White, S.-K. Chiou, L. Rao, P. Sabbatini, and H.-J. Lin Control of p53-dependent Apoptosis by E1B, Bcl-2, and Ha-ras Proteins Cold Spring Harb Symp Quant Biol, January 1, 1994; 59(0): 395 - 402. [Abstract] [PDF]
|
|
|
|
|
|
M Debbas and E White Wild-type p53 mediates apoptosis by E1A, which is inhibited by E1B. Genes & Dev., April 1, 1993; 7(4): 546 - 554. [Abstract] [PDF]
|
|