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International Immunology Advance Access originally published online on February 24, 2009
International Immunology 2009 21(4):443-455; doi:10.1093/intimm/dxp012
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© The Japanese Society for Immunology. 2009. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Cyclical expression of L-selectin (CD62L) by recirculating T cells

Antje Klinger1, Andreas Gebert1, Katja Bieber1, Kathrin Kalies1, Ann Ager2, Eric B. Bell3 and Jürgen Westermann1

1 Center for Structural and Cell Biology in Medicine, Institute of Anatomy, University of Lübeck, Lübeck, Germany
2 Department of Medical Biochemistry and Immunology, Cardiff University, Heath Park, Cardiff, UK
3 Immunology Research Group, University of Manchester, Manchester, UK

Correspondence to: J. Westermann; E-mail: westermann{at}anat.uni-luebeck.de

L-Selectin (CD62L) mediates T-cell entry into lymph nodes. Whether the microenvironment modulates L-selectin expression of T cells during diapedesis and transit is unknown. Therefore, L-selectin expression was determined quantitatively on circulating T cells in blood, lymph nodes and thoracic duct by confocal laser scanning microscopy. We show that in contrast to leukocyte function-associated antigen-1 (CD11a/CD18) and ICAM-1 (CD54), L-selectin expression is cyclically expressed on recirculating T cells. It is reduced to ~30% of the blood value during entry across high endothelial venules. Within lymph nodes, CD4+ T-cell subsets maintain reduced L-selectin expression at a similar level in all compartments (T-cell zone, B-cell zone and medulla). After exit, L-selectin is re-expressed to levels comparable to those of T cells in blood. Apparently, L-selectin levels are not only down-regulated during T-cell activation but also routinely reduced while transmigrating within lymph nodes. L-Selectin down-regulation seems to be ligand independent since it also occurs in the white pulp compartments of the spleen which lack classic L-selectin ligands such as GlyCAM-1 and CD34. In addition, T cells in non-lymphoid organs do not reveal reduced L-selectin levels. Thus, the ability of secondary lymphoid organs to reduce L-selectin expression of T cells prior to activation might be a prerequisite for their characteristic property to induce primary immune responses.

Keywords: CD62L, CLSM, ICAM-1, image analysis, LFA-1, L-selectin, lymph node, migration, organ compartments, rat, T cell

Transmitting editor: T. Hunig

Received 24 July 2008, accepted 27 January 2009.


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