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International Immunology Advance Access originally published online on September 7, 2009
International Immunology 2009 21(11):1225-1237; doi:10.1093/intimm/dxp090
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© The Japanese Society for Immunology. 2009. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Enhanced B cell activation in the absence of CD81

Mrinmoy Sanyal, Rosemary Fernandez and Shoshana Levy

Division of Oncology, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA

Correspondence to: S. Levy; E-mail: slevy{at}stanford.edu

CD81 is a component of the CD19/CD21 co-receptor complex in B cells. However, the role of CD81 in B cell activation has not been clearly elucidated. Here, we demonstrate that Cd81–/– B cells stimulated via their B cell receptor fluxed higher intracellular-free calcium ion along with increased phosphorylation of spleen tyrosine kinase and phospholipase gamma 2. Additionally, Cd81–/– B cells responded to toll like receptor 4 stimulation with increased nuclear factor-kappa B activation, cell proliferation and antibody secretion compared with wild-type B cells. Cd81–/– mice also mounted a significantly higher immune response to T-independent antigens than their wild-type counterparts. Finally, analysis of Cd81–/– B cells that were generated by bone marrow transplantation into Rag1–/– mice confirmed that the hyperactive phenotype is not dependent on the CD81-deficient environment. Taken together, these results indicate that CD81 plays a negative role in B cell activation in vitro and in vivo.

Keywords: B cell activation, calcium mobilization, knockout, signal transduction, tetraspanin, TLR4

Transmitting editor: P. W. Kincade

Received 16 January 2009, accepted 6 August 2009.


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