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International Immunology Advance Access originally published online on January 14, 2008
International Immunology 2008 20(3):317-326; doi:10.1093/intimm/dxm152
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© The Japanese Society for Immunology. 2008. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Tissue-specific expression of B-cell translocation gene 2 (BTG2) and its function in T-cell immune responses in a transgenic mouse model

Rafik Terra1, Hongyu Luo1, Xiaoying Qiao1 and Jiangping Wu1,2

1 The Laboratory of Immunology, Centre hospitalier de l'Université de Montréal, Notre-Dame Hospital, Pavilion DeSève, 1560 Sherbrooke Street East, Montreal, Quebec H2L 4M1, Canada
2 Nephrology Service, Centre hospitalier de l'Université de Montréal, Notre-Dame Hospital, Pavilion DeSève, 1560 Sherbrooke Street East, Montreal, Quebec H2L 4M1, Canada

Correspondence to: J. Wu; E-mail: jianping.wu{at}umontreal.ca

B-cell translocation gene 2 (BTG2) belongs to the anti-proliferative gene family. According to previous in vitro studies, BTG2 overexpression leads to delayed cell cycling. We investigated BTG2 expression during mouse ontogeny and its immune and circadian functions in this study. In situ hybridization showed that BTG2 was expressed at high levels in the central nervous system, liver, stomach, thymus, spleen, skin, adrenal gland, pituitary gland and salivary glands during embryonic days (E10–E17), postnatal days (P1 and P10) and adult stages. Expression was observed in organs and tissues from adult mice with and without a robust proliferation program. Thus, the gene might have important functions that are both related and unrelated to proliferation. BTG2 expression was induced after in vitro T-cell receptor stimulation in T cells using anti-CD3 antibodies. However, transgenic (Tg) mice with actin promoter-driven expression of BTG2 showed normal in vitro and in vivo T-cell responses, such as thymus development, T-cell activation marker expression, T-cell proliferation and migration, as well as in vivo delayed-type hypersensitivity reactions. Although BTG2 was expressed in the suprachiasmatic nucleus and pineal gland in the brain, BTG2 Tg mice had no abnormal circadian behavior. Our data on BTG2 expression during ontogeny provide useful clues for the further investigation of BTG2 function. Additional studies are warranted to examine its role in immune and other systems.

Keywords: BTG2, circadian rhythm, in situ hybridization, T cells, transgenic mouse

Transmitting editor: A. Falus

Received 15 September 2007, accepted 7 December 2007.


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