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International Immunology Advance Access originally published online on January 9, 2008
International Immunology 2008 20(3):307-315; doi:10.1093/intimm/dxm143
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© The Author 2008. Published by Oxford University Press on behalf of The Japanese Society for Immunology. All rights reserved.
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CD4+CD25+ regulatory T cells in the small intestinal lamina propria show an effector/memory phenotype

Zijin Guo1,*, Myoung Ho Jang1,2,*, Kazuhiro Otani1, Zhongbin Bai1, Eiji Umemoto1, Masanori Matsumoto1,3, Mika Nishiyama1, Mikako Yamasaki1, Satoshi Ueha4, Kouji Matsushima4, Takako Hirata3 and Masayuki Miyasaka1,5

1 Laboratory of Immunodynamics, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan
2 Laboratory of Gastrointestinal Immunology, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan
3 The 21st Century Center of Excellence Program, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
4 Department of Molecular Preventive Medicine and SORST, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
5 Laboratory of Immunodynamics, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan

Correspondence to: M. Miyasaka; E-mail: mmiyasak{at}orgctl.med.osaka-u.ac.jp

CD4+CD25+ regulatory T cells (Tregs) have been implicated in the suppression of pathogenic responses to both self- and non-self-antigens in the intestine. However, their precise properties and functions in the gut, as well as the molecular basis of their recruitment to the gut, are poorly understood. Here, we found that most of the CD4+CD25+ T cells in the small intestinal lamina propria (LP) express Foxp3 and exhibit an ‘effector/memory’ phenotype, CD44hiCD45RBloCD62L–, whereas only a minority of the Foxp3+CD4+CD25+ T cells in the spleen and mesenteric lymph nodes showed this phenotype. The Tregs in the small intestinal LP (LP-Tregs) expressed higher levels of CCR4 and CCR9 and a substantially lower level of CCR7 than the Tregs in the spleen. In vitro, the LP-Tregs showed chemotaxis to CCL25/thymus-expressed chemokine. In addition, they showed efficient chemotaxis to the CCR4 ligands, CCL17/thymus and activation-regulated chemokine and CCL22/macrophage-derived chemokine, which are abundantly expressed by dendritic cells (DCs) in the small intestinal LP. In vivo, ~50% of the LP-Tregs were closely associated or in direct contact with LP-DCs. These findings demonstrate that LP-Tregs are phenotypically and functionally unique and raise the possibility that they are retained in the small intestinal LP through the action of CCL17 and CCL22, which are locally produced by LP-DCs.

Keywords: cell migration, chemokines, dendritic cells, mucosal immunity, regulatory T cells

* These authors contributed equally to this study.

Transmitting editor: T. Saito

Received 4 June 2007, accepted 7 December 2007.


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