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International Immunology Advance Access originally published online on September 15, 2008
International Immunology 2008 20(11):1451-1456; doi:10.1093/intimm/dxn102
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© The Japanese Society for Immunology. 2008. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Mass spectrometric identification of an HLA-A*0201 epitope from Plasmodium falciparum MSP-1

Jean-Philippe Carralot*, Claudia Lemmel*, Stefan Stevanovic and Steve Pascolo

Department of Immunology, Institute for Cell Biology, University of Tübingen, Auf der Morgenstelle 15, D-72076 Tübingen, Germany

Correspondence to: S. Pascolo; E-mail: steve.pascolo{at}uni-tuebingen.de

Cytotoxic T lymphocytes (CTL) directed against Plasmodium falciparum-derived antigens were shown to play an important role for the protection against malaria. Although several CTL epitopes have been identified from P. falciparum sporozoite-derived antigens, none has been described for the merozoite form. Since the merozoite surface protein (MSP)-1 is a known target of the immune response, we focused on this protein to identify HLA-A*0201-associated epitopes. Using our mass spectrometry-based method [the ‘predict–calibrate–detect’ (PCD) approach], we were able to identify an MSP-1-derived epitope in the peptide mixture naturally associated with HLA-A*0201 molecules purified from an MSP-1-expressing cell line. CTLs against this epitope were generated from HLA-A*0201 monochain transgenic mice (HHD). They specifically killed MSP-1-expressing HLA-A2-positive target cells. Thus, we describe here the first MHC class I epitope from the merozoite form of P. falciparum. This epitope can be used as a tool for the immunomonitoring of natural or vaccine-induced CTL immune responses against malaria and could eventually be proposed as a component of an anti-malaria peptide-based vaccine.

Keywords: Plasmodium falciparum, MSP-1, epitope, mass spectrometry, HLA transgenic mice

* These authors contributed equally to this study.

Transmitting editor: A. Radbruch

Received 10 December 2007, accepted 18 August 2008.


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