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International Immunology, Vol. 2, No. 9, pp. 869-877,September 1990
© 1990 Japanese Society for Immunology

Neonaltal mouse CD4+ mature thymocytes show responsiveness to interleuking 2 and interleukin 7: growth in vitro of negatively selected Vß 6- and Vß 11-expressing CD4+ cells from (C57BL/6xDBA/2)F1 mice

Rhodri Ceredig and Caroline Waltzinger

Laboratoire de Génétique Moléculaire des Eucaryotes, CNRS, Unité 184 de Biologique, Molé et de Génie Génétique de I'INSERM, Institut de Chimle Biologique, Faculté de Médenine, 11 rue Humann, 67085 Strasbourg Cédex, France

Correspondence to: Correspondence to R.ceredig

By three colour flow microfluorimetry, we have recently shown that neonatal mouse CD4 single positive thymocytes are a population of proliferating cells. Furthermore, analysis of CD4 + thymocytes from (C57BL/6 x DBAx2)F1 mice showed that they proliferate regardless of whether they express particular vyencoded TCR molecules (Vß6 and Vß11) that are undergoing Intrathymic deletion. In this report, cell culture experiments demonstrate that unstimulated neonatal CD4+ thymocytes from such mice proliferate in vitro In response to a combination of r-IL-2 and r-IL-7. Simultaneous three colour analysis of Vs TCR, CD4 expression, and DNA content of these cultured cells shows that Vß6+, -8+, and -11+ cells grow equally well. Experiments where cells were cultured overnight in unsupplemented medium did not reveal preferential loss of negatively selected (Vß6+ and Vß11+) subpopulations of CD4+ cells. Taken together, these results suggest that IL-2 and IL-7 play a role In the Intrathymic proliferation of developing mature T cells.

Keywords: T cell development, clonal deletion, flow microfluonmetry

Received 9 May 1990, accepted 5 June 1990.


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