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International Immunology, Vol. 2, No. 9, pp. 813-819,September 1990
© 1990 Japanese Society for Immunology

Somatic mutations in antibodies expressed by germinal centre B cells early after primary immunization

Michael Apel and Claudia Berek

Institute for Genetics, University of Cologne Weyertal 121, D-5000 Köln 41, FRG

Correspondence to: Correspodence to: Clauda Berek

We have studied the maturation of the immune response by looking at the generation of antibody diversity in germinal centre B cells. Mice were immunized with the antigen 2-phenyloxazolone. Germinal centre B cells, defined by their strong binding to peanut agglutinin (PNAhhl), were sorted from the spleen and fused. Ten days after imrnunization high numbers of antigen specific hybridoma lines were obtained from the PNAM subset of B cells, suggesting that the small fraction of B cells which are PNAhl harbour the antigen activated population. The majority of the day 10 quences from PNAhhl cells were shown to be mutated. However, in contrast to results from later stages of the lmmune response, most of the mutations found were silent. The preferential expansion of B cell clones expressing the mutations characteristic of the mature response was not observed at this stage. Among these hybridoma lines was at least one which, apparentty through somatic mutation, had lost the ability to bind antlgen. We conclude that in the micro-environment of the germinal centre the B cell repertoire is diversified by hypermutation.

Keywords: antibody diversity, germinal centers, somatic hypermutation, matuuration of the immune response

Received 11 April 1990, accepted 4 June 1990.


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