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International Immunology, Vol. 2, No. 8, pp. 725-733,August 1990
© 1990 Japanese Society for Immunology

Cell division-associated expression of an epitope, KH17, on early developing thymocytes

Kunio Sano, Hidehiro Kishimoto, Hajime Karasuyama, Yoshihiro Asano and Tomio Tada

Department of Immunology, Faculty of Medicine, University of Tokyo Toyko, Japan

Correspondence to: Correspondence to. K. Sano, as above

A newly established monocional antibody, KH17, detects a unique epitope temporarily expressed on early developing CD3– thymocytes confined to a cycling stage. KH17 is detectable on a part of CD4–CD8–, CD4–CD8+, and CD4+CD8+ cells, but not on CD4+CD8– thymocytes. By four-color flow cytometry analysis using KH17, we were able to define the heterogeneity of immature CD4–CD8– thymocytes by the expression of KH17 and IL-2R. In Thy-1-congeneic bone marrow chimeras, the appearance of KH17-IL-2R+ thymocytes preceded the increase of KH17+ IL-2R– cells. The antibody could also divide CD3–CD4–CD8+ cells into two subpopulations, KH17+ and KH17–, which showed a continuum. In the fetal thymus there was a rapid and dramatic increase of KH17–CD4+CD8+ thymocytes concomitant with a decrease of KH17+CD4–CD8+ thymocytes in later gestation days. KH17 is not expressed on resting peripheral T cells, but is expressed on a large proportion of Con A-activated blastic spleen cells. The KH17 molecules precipitated from Con A-activated spleen cells were 55 and 75 kd polypeptides, but different from IL-2R subunits.

Keywords: flow cytometry, thymus, T cell development

Received 23 February 1990, accepted 20 May 1990.


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