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International Immunology, Vol. 2, No. 6, pp. 563-570,June 1990
© 1990 Japanese Society for Immunology

Dendritic cells support production of IgA and other non-IgM isotypes in clonal microculture

Carol E. Schrader, Anna George1, Roy L. Kerlin2 and John J. Cebra

Department of Biology, University of Pennsylvania Philadelphia, PA 19104-6018, USA

Correspondence to: Correspondence to: Dr John J. Cebra, as above

Microcultures of helper T (Th) cells and a few appropriately primed murine B cells can be used to detect cognate T-B Interactions which lead to clonal production of IgM, lgG1, and IgE. However, lgG2, lgG3, and IgA are very rarely expressed. We have found that the addition of dendritic cells to such cultures creates an extremely supportive environment for clones expressing IgA with other isotypes, as well as clones expressing only detectable IgA. Typically, 400 dendritic cells were added to 3000 conalbumln-speclflc Th cells (D10.G4.1) and 30 hapten-speciflc Peyer’s patch (PP) B cells with antigen in 15 µl. The response was antigen dependent and clonal. Almost half of the clones expressed only non-IgM isotypes, 43% expressed some IgA, and 14% expressed some lgG3; isotype diversity Increased over time. Dendritic cells from PP and spleen were found to be equally supportive, and allowed the number of T cells required In microculture to be decreased from 3000 to 400. However, T cell proliferation was not required for the supportive effect of dendritic cells. Surface IgD-bearing cells were also found to switch to IgA production in microculture as judged by their generating clones expressing IgM along with IgA and other Isotypes. Again, IgA was usually expressed only In the presence of dendritic cells. The mechanism may Involve dendritic cell-induced T cell activation and/or dendritic cell factors, and Is under investigation.

Keywords: dendritic cells, Payer's patch

1Current address: Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 ONN, UK

2Current address: CSIRO, Division of Tropical Animal Production, Long Pocket Laboratories, PO Indooroopilly, Brisbane, Queensland 4068, Australia

Received 8 January 1990, accepted 22 March 1990.


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