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International Immunology, Vol. 2, No. 5, pp. 435-441,May 1990
© 1990 Japanese Society for Immunology

Peptides recognized by class I restricted T cells also bind to MHC class II molecules

J. K. Hickling1,, C. M. Fenton, K. Howland, S. G. E. Marsh2 and J. B. Rothbard1

Laboratory of Molecular Immunology, Imperial Cancer Research Fund PO Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK
2 Tissue Antigen Laboratory, Imperial Cancer Research Fund PO Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK

Correspondence to: Correspodence to: J.K. Hicking, see Present address

The mechanisms of antigen recognition employed by both class I and class II MHC-restricted T cells are very similar, yet many of the T cell determinants described to date are recognized in the context of a single class of MHC molecules, and generally with only one or a very few different MHC alleles. To determine whether this might be due to a structural difference between class I and class II restricted T cell determinants, peptides previously shown to be recognized in the context of MHC class I proteins by mouse or human CD8+ T lymphocytes were tested for their capacity to bind to HLA-DR molecules on the surface of B lymphoblastoid cell lines (B-LCL). Four out of five class I restricted T cell determinants tested bound to a panel of B-LCL, and the binding was inhibited by anti-HLA-DR mAb. The peptides did not bind to the class Il-negative B-LCL RJ2.2.5 nor to mouse L cells, but did bind to L cells transfected with HLA-DR1.

Keywords: MHC restriction, peptide binding, flow cytometry

1Present address: Immulogic Pharmaceutical Corporation, 855 California Avenue, Palo Alto, CA 94304, USA

Received 30 December 1989, accepted 26 February 1990.


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