International Immunology, Vol. 2, No. 5, pp. 419-425,May 1990
© 1990 Japanese Society for Immunology
Analysis of recent thymic emigrants with subset- and maturity-related markers
The Walter and Eliza Hall Institute of Medical Research Melbourne, Victoria 3050, Australia
Correspondence to: Correspodence to: Katherine A Kelly, as above
The thymus plays an integral role in the development and production of T lymphocytes.However, thymocytes differ markedly in their phenotypic characteristics from the T cells normally found in the peripheral lymphoid organs. We have examined the phenotypic characteristics of recent thymic emigrants and compared them with both mature phenotype thymocytes (CD4© CD8-CD3 + and CD4-CD8©CD3 +) and lymph node T cells. Recent thymic emigrants were defined as those fluorescein-positive cells found in the lymph node up to 16 h after intrathymic injection of fluorescein. Most cells emigrating from the thymus expressed CD and either CD4 or CD8, indicating maturity. Recent thymic emigrants, like mature phenotype thymocytes, were slightly larger on average than peripheral T cells, but this differential was lost within 24 h of emigration. Also like mature thymocytes but unlike peripheral T cells, some recent emigrants expressed heat-stable antigen. This did not change within 24 h of emigration. The antigen CD44 (Pgp-1, Ly-24) was expressed on a proportion of mature thymocytes, recent thymic emigrants, and peripheral T cells, and its expression did not show any clear relationship to maturity. The antigen CD45R also did not show marked changes associated with maturity, but our data do not parallel the published data of the expression of CD45R in the human. We conclude that recent thymic emigrants are phenotypically mature with respect to some antigens but not others. None of the antigens we investigated could have been used to uniquely distinguish recent thymic emigrants from peripheral T cells or from mature thymocytes
Keywords: S-protein, vitronectin
Received 14 November 1989, accepted 26 February 1990.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  N. H. E. Mabarrack, N. L. Turner, and G. Mayrhofer Recent thymic origin, differentiation, and turnover of regulatory T cells J. Leukoc. Biol., November 1, 2008; 84(5): 1287 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hu and A. August Naive and Innate Memory Phenotype CD4+ T Cells Have Different Requirements for Active Itk for Their Development J. Immunol., May 15, 2008; 180(10): 6544 - 6552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Posevitz, B. Arndt, T. Krieger, N. Warnecke, B. Schraven, and L. Simeoni Regulation of T Cell Homeostasis by the Transmembrane Adaptor Protein SIT J. Immunol., February 1, 2008; 180(3): 1634 - 1642. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. McCaughtry, M. S. Wilken, and K. A. Hogquist Thymic emigration revisited J. Exp. Med., October 29, 2007; 204(11): 2513 - 2520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Clise-Dwyer, G. E. Huston, A. L. Buck, D. K. Duso, and S. L. Swain Environmental and Intrinsic Factors Lead to Antigen Unresponsiveness in CD4+ Recent Thymic Emigrants from Aged Mice J. Immunol., February 1, 2007; 178(3): 1321 - 1331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Uldrich, S. P. Berzins, M. A. Malin, P. Bouillet, A. Strasser, M. J. Smyth, R. L. Boyd, and D. I. Godfrey Antigen challenge inhibits thymic emigration. J. Immunol., April 15, 2006; 176(8): 4553 - 4561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Savino, D. A. Mendes-da-Cruz, S. Smaniotto, E. Silva-Monteiro, and D. M. S. Villa-Verde Molecular mechanisms governing thymocyte migration: combined role of chemokines and extracellular matrix J. Leukoc. Biol., June 1, 2004; 75(6): 951 - 961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Feng, K. J. Woodside, B. A. Vance, D. El-Khoury, M. Canelles, J. Lee, R. Gress, B. J. Fowlkes, E. W. Shores, and P. E. Love A potential role for CD69 in thymocyte emigration Int. Immunol., June 1, 2002; 14(6): 535 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Le Campion, C. Bourgeois, F. Lambolez, B. Martin, S. Leaument, N. Dautigny, C. Tanchot, C. Penit, and B. Lucas Naive T cells proliferate strongly in neonatal mice in response to self-peptide/self-MHC complexes PNAS, April 2, 2002; 99(7): 4538 - 4543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. M. Raaphorst, A. P. Otte, F. J. van Kemenade, T. Blokzijl, E. Fieret, K. M. Hamer, D. P. E. Satijn, and C. J. L. M. Meijer Distinct BMI-1 and EZH2 Expression Patterns in Thymocytes and Mature T Cells Suggest a Role for Polycomb Genes in Human T Cell Differentiation J. Immunol., May 15, 2001; 166(10): 5925 - 5934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-k. Lee, K. Kim, L. A. Welniak, W. J. Murphy, K. Muegge, and S. K. Durum Thymic emigrants isolated by a new method possess unique phenotypic and functional properties Blood, March 1, 2001; 97(5): 1360 - 1369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. S. Huang, M. Hahne, M. Schroeter, K. Frei, A. Fontana, A. Villunger, K. Newton, J. Tschopp, and A. Strasser Activation of Fas by FasL induces apoptosis by a mechanism that cannot be blocked by Bcl-2 or Bcl-xL PNAS, December 21, 1999; 96(26): 14871 - 14876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. De Boer and A. J. Noest T Cell Renewal Rates, Telomerase, and Telomere Length Shortening J. Immunol., June 15, 1998; 160(12): 5832 - 5837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Tough and J. Sprent Lifespan of gamma /delta T Cells J. Exp. Med., February 2, 1998; 187(3): 357 - 365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-S. Teh, B. Motyka, and S.-J. Teh Positive Selection of Thymocytes Expressing the Same TCR by Different MHC Ligands Results in the Production of Functionally Distinct Thymocytes Distinguished by Differential Expression of the Heat Stable Antigen J. Immunol., January 15, 1998; 160(2): 718 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Chambers, D. Cado, T. Truong, and J. P. Allison Thymocyte development is normal in CTLA-4-deficient mice PNAS, August 19, 1997; 94(17): 9296 - 9301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Sprent and D. Tough Lymphocyte life-span and memory Science, September 2, 1994; 265(5177): 1395 - 1400. [Abstract] [PDF]
|
|
|
|
|
|
A. Le Campion, C. Bourgeois, F. Lambolez, B. Martin, S. Leaument, N. Dautigny, C. Tanchot, C. Penit, and B. Lucas Naive T cells proliferate strongly in neonatal mice in response to self-peptide/self-MHC complexes PNAS, April 2, 2002; 99(7): 4538 - 4543. [Abstract] [Full Text] [PDF]
|
|