Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kanagawa, O.
Right arrow Articles by Takagaki, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kanagawa, O.
Right arrow Articles by Takagaki, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

International Immunology, Vol. 2, No. 10, pp. 957-964,October 1990
© 1990 Japanese Society for Immunology

Expression and function of the tranfected CD8{alpha} chain in murine T cell hybridomas

Osami Kanagawa, Hiromitsu Nakauchi1, Rafick P. Sekaly2, Keiji Maeda3 and Yotaroh Takagaki3

Department of Pathology and Medicine, Washington University St Louis, MO, USA
1 The Insitute of Physical and Chemical Research Tsukuba, Japan
2 Laboratory of Molecular Immunology, Clinical Research Insitute of Montreal Montreal, Canada
3 Howard Hughes Medical Insitute and Center for Cancer Research and Department of Biology, Massachusetts Insitute of Technology Cambidge, MA, USA

Correspondence to: Correspondence to: O. Kanagawa, Washington University School of Medicine, Department of Phathology, Box 8118, 660 South Euclid Avenue, St Louis, MO 63110, USA

Expression and function of mouse and human CD8 (mCD8 and hCD8) {alpha} chain molecules In mouse T cell hybridomas were analyzed. The expression of cytolytic T lymphocyte-derived CD8 molecules was supprmsd In hybridomas established by fusing the BW5147 thymoma to a CD8+ cytolytic T lymphocyte clone, while expression of CD4 remained intact In BW x CD4+ helper T cell hybridomas. However, hybridomas established by tusing a cytolytlc T cell clone wtth BW5147 cell lines, transfected wlth either the mCD8{alpha} or hCD8{alpha} chaln, expressed the T cell-derived mCD8ß chain as a CD8 heterodimer (mCD8{alpha}/mCD8{alpha} or hCD8{alpha}/mCD8ß). These data suggest that negative regulatory mechanlsms for the mCD8{alpha} gene In BW thymoma failed to suppress mCD8ß gene expression, indicating different regulatory mechanisms for the tightly linked mCDh and mCD8ß genes. Analysis of the antlgen reactivity of the hybridomas revealed that the human CDB{alpha} chain felled to increase the mouse T cell receptor - class I MHC Interaction, even as a heterodimeric form with mCD8ß8 molecules. However, both the human mCD8{alpha} homodimer and the heterodimeric form with mCD88 were found to be capable of suppressing the class 11-restricted T cell response.

Keywords: CD8{alpha}, CD8ß, transfection, antigen specificity

Received 22 March 1990, accepted 29 June 1990.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
C.-Y. Huang and O. Kanagawa
Ordered and Coordinated Rearrangement of the TCR {{alpha}} Locus: Role of Secondary Rearrangement in Thymic Selection
J. Immunol., February 15, 2001; 166(4): 2597 - 2601.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.