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International Immunology Advance Access originally published online on March 15, 2007
International Immunology 2007 19(5):591-607; doi:10.1093/intimm/dxm024
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© The Japanese Society for Immunology. 2007. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Cross-linking a mAb to NKR-P2/NKG2D on dendritic cells induces their activation and maturation leading to enhanced anti-tumor immune response

Raghvendra M. Srivastava, Ch. Varalakshmi and Ashok Khar

Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India

Corresponding to: A. Khar; E-mail: khar{at}ccmb.res.in

NKR-P2/NKG2D is the chief tumor recognition receptor of NK cells and some T cells, which recognizes stress inducible ligands on tumors and mediates cell activation. We have recently reported the involvement of NKR-P2 in rat dendritic cell (DC) activation. We demonstrate the potential of agonistic anti-NKR-P2 mAb (1A6), which mimics the NKR-P2 ligand and induces activation and maturation of DCs. Interaction of DCs with 1A6 enhances nitric oxide-mediated apoptosis in tumor cells. Cross-linking of NKR-P2 with mAb1A6 up-regulates MHC II, CD86, CD1a, antigen-presentation function and decreases endocytic activity of DC, thus drives DCs to play a pivotal role in adaptive immune responses. NKR-P2 cross-linking with 1A6 also induced the secretion of inflammatory cytokines, IL-1ß, tumor necrosis factor-{alpha}, IFN-{gamma} and IL-12 by DCs. Blocking of 1A6-mediated activation and maturation with inhibitors of PI3K, p38K and ERK1/2K suggests involvement of MAP kinase in signal transduction. 1A6 cross-linking activates nuclear factor kappa B, which acts as key executioner of DC activation. Administration of 1A6 antibody induces rapid regression and protective immune responses against transplantable tumors, suggesting mAb induced activation and maturation of DCs, leading to enhanced anti-tumor immune response.

Keywords: apoptosis, dendritic cells, maturation, NKR-P2/NKG2D, nitric oxide

Transmitting editor: W. Yokoyama

Received 8 August 2006, accepted 7 February 2007.


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