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International Immunology Advance Access originally published online on February 27, 2007
International Immunology 2007 19(4):545-556; doi:10.1093/intimm/dxm020
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© The Japanese Society for Immunology. 2007. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Antibody class switching differs among SJL, C57BL/6 and 129 mice

Denise A. Kaminski1,2 and Janet Stavnezer1

1 Immunology and Virology Program, Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
2 Present address: Trudeau Institute, Saranac Lake, NY 12983, USA

Correspondence to: J. Stavnezer; E-mail: janet.stavnezer{at}umassmed.edu

Inbred mouse strains used for gene manipulation studies vary in many respects, including immune system function. These differences can interfere with data interpretation unless the mice are well backcrossed. Here, we show that antibody class switching to IgG3 in cultured splenic B cells from Swiss James Lambert (SJL) and 129/Sv mice is 2- to 6-fold less efficient compared with C57BL/6 (B6). Under optimal stimulation conditions, IgA switching is also 2- to 19-fold lower in SJL and 129/Sv B cells. Splenic B cells from SJL mice express higher levels of CD19 and CD21 compared with B6, and their CD21highCD23low B cells have little CD9 expression, suggesting atypical marginal zone (MZ) B cells. However, sort purification of splenic B cell subsets did not equalize in vitro class switching to IgG3 or IgA between SJL and B6. 129/Sv spleens have a 3-fold greater number of MZ B cells compared with B6, with similar CD9 expression. Poor IgG3 switching by 129/Sv B cells is specific to CD23high follicular B cells, whereas similar changes in IgA switching are seen in both CD21high and CD23high B cell subsets from 129/Sv. Therefore, the functions and phenotypes of mature B cells differ among three common strains of mice.

Keywords: B cells, inbred mouse strains, isotype switch

Transmitting editor: M. Nussenzweig

Received 1 September 2006, accepted 27 January 2007.


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