Skip Navigation


International Immunology Advance Access originally published online on October 3, 2007
International Immunology 2007 19(11):1281-1289; doi:10.1093/intimm/dxm098
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
19/11/1281    most recent
dxm098v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Unsoeld, H.
Right arrow Articles by Pircher, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Unsoeld, H.
Right arrow Articles by Pircher, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© The Japanese Society for Immunology. 2007. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Abrogation of CCL21 chemokine function by transgenic over-expression impairs T cell immunity to local infections

Heike Unsoeld1, Katja Mueller1, Ulrike Schleicher2, Christian Bogdan2, Jörg Zwirner3, David Voehringer4 and Hanspeter Pircher1

1 Department of Immunology, Institute of Medical Microbiology and Hygiene, University of Freiburg, Freiburg, Germany
2 Department of Medical Microbiology and Hygiene, University of Freiburg, Freiburg, Germany
3 Department of Immunology, University of Göttingen, Göttingen, Germany
4 Institute for Immunology, Ludwig Maximilian University, Munich, Germany

Correspondence to: H. Pircher; E-mail: hanspeter.pircher{at}uniklinik-freiburg.de

The CC chemokine receptor 7 (CCR7) and its two ligands, CCL21 and CCL19, play an important role in migration of immune cells to lymphoid tissue. To analyze the function of CCR7 in T cell immunity to infectious agents in vivo, transgenic (tg) mice expressing CCL21 in an ubiquitous fashion were generated. These mice contained high amounts of CCL21 in the serum (~0.3 µg/ml that resulted in CCR7 down-regulation and in a strongly impaired migration of T cells toward CCL21 in vitro. Lymph nodes in CCL21-tg mice were reduced in size but with intact microanatomy and normal distribution of T and B cells. CCL21-tg mice showed a significantly decreased CD8 T cell response to lymphocytic choriomeningitis virus after footpad infection, whereas the response after systemic infection was not altered. Likewise, the CD4 T cell response to footpad infection with Leishmania major was considerably lowered and CCL21-tg mice failed to clear parasites from infected skin. Taken together, these data demonstrate the importance of CCR7 in mediating T cell immunity to viral and parasitic pathogens after local infection.

Keywords: cell trafficking, in vivo desensitization, transgenic mice

Transmitting editor: H. Robson Mac Donald

Received 19 April 2007, accepted 10 August 2007.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.