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International Immunology Advance Access originally published online on August 14, 2007
International Immunology 2007 19(10):1165-1173; doi:10.1093/intimm/dxm086
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© The Japanese Society for Immunology. 2007. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Imaging CD8+ T cell dynamics in vivo using a transgenic luciferase reporter

Mitra Azadniv1, Kari Dugger2, William J. Bowers3, Casey Weaver2,* and Ian N. Crispe1,*

1 David H Smith Center for Microbiology and Immunology, Aab Institute for Biomedical Research, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
2 Department of Pathology, University of Alabama at Birmingham, 845 19th Street South, 870 Bevill Biomedical Research Building, Birmingham AL 35294, USA
3 Department of Neurology, Center for Aging and Developmental Biology, Aab Institute for Biomedical Research, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA

Correspondence to: I. N. Crispe; E-mail: nick_crispe{at}urmc.rochester.edu

After activation, populations of antigen-specific T cells flow between sites of antigen expression, local lymphoid structures and other lymphoid and non-lymphoid organs. In this study, we documented the in vivo dynamics of a CD8+ T cell response to antigen delivered using herpes simplex virus amplicon vectors and revealed several unexpected features. First, the T cells localized to the site of vector injection, as well as the draining lymph node within 24–48 h. Second, the major site to which T cells later redistributed were intra-abdominal lymphoid organs, including milky spots, mesenteric and lumbar lymph nodes. We determined the relationship between bioluminescent signal and antigen-specific T cell numbers in various lymphoid organs, and concluded that bioluminescent signal is a valid surrogate measure of T cell abundance in superficial lymph nodes, but not in deeper structures such as the spleen.

Keywords: bioluminescence, luciferase

* These authors contributed equally to this Study.

Transmitting editor: K. M. Murphy

Received 17 January 2007, accepted 3 July 2007.


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