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International Immunology Advance Access originally published online on December 6, 2006
International Immunology 2007 19(1):99-103; doi:10.1093/intimm/dxl126
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© The Japanese Society for Immunology. 2006. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Synergy between LPS and immobilized anti-human CD3{epsilon} mAb for activation of cord blood CD3+ T cells

Michael R. Goldberg1,2, Noemi Luknar-Gabor1, Galia Zadik-Mnuhin2, Penina Koch1, Joseph Tovbin3 and Yitzhak Katz1,2

1 Institute of Allergy and Immunology, Assaf Harofeh Medical Center, Zerifin 70300, Israel
2 Department of Pediatrics, Assaf Harofeh Medical Center, Zerifin 70300, Israel
3 Department of Obstetrics and Gynecology, Assaf Harofeh Medical Center, Zerifin 70300, Israel

Correspondence to: M. R. Goldberg; E-mail: goldbergsm{at}yahoo.com

Despite an attenuated proliferative response in human cord blood mononuclear cells (CBMCs) to activation of its TCR in vitro, the neonate is capable of mounting a mature Th1-type response to BCG vaccination. We hypothesized that in the context of other innate triggers, activation of the TCR can be restored. In order to test this hypothesis, we analyzed CBMC response to LPS, with LPS serving as a surrogate activator of the innate system. We performed proliferative assays on 34 maternal–neonatal pairs of PBMCs and CBMCs, respectively. In all, 30/34 (88%) of CBMCs proliferated in response to LPS (10 µg ml–1, P < 2.7 x 10–7), in contrast to only 10/32 (31%) of their respective maternally derived PBMCs, despite having a comparatively greater response to PHA than did their CBMC counterparts (P < 0.0002). LPS synergized with immobilized anti-human CD3{epsilon} mAb (1.25–10 µg ml–1) to augment the proliferative response in CBMCs but failed to do so in maternally derived PBMCs. LPS responsiveness and its synergy with activation of the TCR in CBMCs were independent of accessory cells. These results are the first evidence that LPS and anti-CD3 mAb are synergistic, demonstrating a critical link between the innate and adaptive immune systems.

Keywords: cord blood mononuclear cells, lipopolysaccharide, T cell receptor

Transmitting editor: D. Wallach


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 Am. J. Respir. Cell Mol. Bio.Home page
M. R. Goldberg, O. Nadiv, N. Luknar-Gabor, G. Zadik-Mnuhin, J. Tovbin, and Y. Katz
Correlation of Th1-Type Cytokine Expression and Induced Proliferation to Lipopolysaccharide
Am. J. Respir. Cell Mol. Biol., June 1, 2008; 38(6): 733 - 737.
[Abstract] [Full Text] [PDF]


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