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International Immunology Advance Access originally published online on December 22, 2005
International Immunology 2006 18(2):259-267; doi:10.1093/intimm/dxh365
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© The Japanese Society for Immunology. 2005. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Lymphoid B cells induce NF-{kappa}B activation in high endothelial cells from human tonsils

Rodrigo Naves1,4,*, Lilian I. Reyes3,4,*, Mario Rosemblatt3,4,5, Sergio Jacobelli1, Alfonso González1,2,4 and María R. Bono3,4

1 Departamento de Inmunología Clínica y Reumatología, Facultad de Medicina, Pontificia Universidad Católica de Chile, Casilla, Santiago 1365, Chile
2 Centro FONDAP de Regulación Celular y Patología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, Santiago, Chile
3 Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Casilla 635, Santiago, Chile
4 Millennium Institute for Fundamental and Applied Biology (MIFAB), Zañartu 1482, Santiago, Chile
5 Fundación Ciencia para la Vida and Universidad Andrés Bello, Santiago Chile, AV Zañartu 1482, Santiago, Chile

Correspondence to: M. Rosemblatt; E-mail: mrosembl{at}bionova.cl

Immune surveillance depends on still poorly understood lymphocyte–endothelium interactions required for lymphocyte transendothelial migration into secondary lymphoid organs. The nuclear factor {kappa}B (NF-{kappa}B) regulatory system and its inhibitory I{kappa}B proteins control the inducible expression of adhesion molecules, cytokines and chemokines involved in endothelial activation and lymphocyte transmigration. Here we present results showing the activation of this system in response to the interaction of high endothelial cells from human tonsils (HUTEC) with human B and T lymphoid cell lines and primary tonsillar lymphocytes. Western blot and electrophoretic mobility shift assays show that adhesion of different lymphoid cells induce varying levels of NF-{kappa}B activation in HUTEC, with Daudi cells, tonsil-derived B cell line 10 (TBCL-10) and primary tonsillar B lymphocytes causing the strongest activation. The main NF-{kappa}B protein complexes translocated to the nucleus were p65/p50 and p50/p50. Results from reverse transcription–PCR and flow cytometry analysis of HUTEC indicate that the interaction with Daudi cells induce an increased expression of IL-6 and IL-8 mRNA and cell-surface expression of intercellular adhesion molecule-1, all of which were prevented by sodium salicylate, an inhibitor of NF-{kappa}B activation. Transwell experiments show that NF-{kappa}B activation and the response of HUTEC to the interaction of Daudi cells does not depend on direct cell–cell contact but rather on the production of soluble factors that require the presence of both cell types. These results suggest that lymphocytes and high endothelium establish a cross talk leading to NF-{kappa}B-mediated expression of cytokines and adhesion molecules, inducing endothelial cell activation.

Keywords: cytokines, endothelial activation, high endothelium, NF-{kappa}B

Transmitting editor: G. Hammerling

* These authors contributed equally to this work.


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