Skip Navigation


International Immunology Advance Access originally published online on December 16, 2005
International Immunology 2006 18(1):19-29; doi:10.1093/intimm/dxh338
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
18/1/19    most recent
dxh338v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Yao, G.
Right arrow Articles by Sun, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yao, G.
Right arrow Articles by Sun, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© The Japanese Society for Immunology. 2005. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling

Gang Yao1, Weiyan Chen2, Haibin Luo3, Qunfeng Jiang1, Zongxiang Xia2,*, Lei Zang1, Jianping Zuo3, Xin Wei4, Zhengjun Chen1, Xu Shen3, Chen Dong5 and Bing Sun1,6,*

1 Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
2 State Key Laboratory of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China
3 Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China
4 GL Biochem (Shanghai) Ltd, 351 Guo Shoujing Road, Shanghai 201203, China
5 Department of Immunology, MD Anderson Cancer Center, 7455 Fannin, Unit 902, Houston, TX 77030-1903, USA
6 Immunology Division, E-Institutes of Shanghai Universities

* Correspondence to: B. Sun; E-mail: bsun{at}sibs.ac.cn, Zx. Xia; E-mail: xiazx{at}mail.sloc.ac.cn

Murine IL-4 is a pleiotropic cytokine with undefined core functional region for eliciting downstream signaling. We used molecular modeling to predict the binding sites recognized by an anti-IL-4-neutralizing mAb (11B.11) and peptide phage display to delineate their makeup. The results of these approaches were confirmed by site-directed mutagenesis analysis. The results suggest that the amino acid residues spanning from 79 to 86 (QRLFRAFR) on IL-4 are of the major binding site for 11B.11. Furthermore, the functional experiments demonstrate that the residues R80, R83 and R86, which are located in the helix C of murine IL-4, play a crucial role in binding to the IL-4R {alpha}-chain. Taken together, a new core functional region of murine IL-4 is identified, which provides new insight into the interaction between IL-4 and IL-4R{alpha}. In addition, the results demonstrate that 11B.11 binds to a core functional region of murine IL-4, which prevents this cytokine from interacting with its cognate receptor.

Keywords: 11B.11, core functional region of IL-4, IL-4, molecular modeling, peptide phage display

Transmitting editor: Dr J. Allison


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.