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International Immunology Advance Access originally published online on August 15, 2005
International Immunology 2005 17(9):1249-1255; doi:10.1093/intimm/dxh301
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© The Japanese Society for Immunology. 2005. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org

IgE- and Fc{varepsilon}RI-mediated migration of human basophils

Maho Suzukawa1, Koichi Hirai2, Motoyasu Iikura1, Hiroyuki Nagase3, Akiko Komiya1, Chitose Yoshimura-Uchiyama1,4, Hirokazu Yamada1, Chisei Ra5, Ken Ohta3, Kazuhiko Yamamoto1 and Masao Yamaguchi1

1 Department of Allergy and Rheumatology and 2 Department of Bioregulatory Function, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan
3 Department of Respiratory Medicine, University of Teikyo School of Medicine, Tokyo, Japan
4 Department of Pediatrics, University of Tokyo Graduate School of Medicine, Tokyo, Japan
5 Division of Molecular Cell Immunology and Allergology, Nihon University Graduate School of Medical Sciences, Tokyo, Japan

Correspondence to: M. Yamaguchi; E-mail: myama-tky{at}umin.ac.jp

Local accumulation of basophils at inflammatory sites is observed in experimental antigen challenge and in allergic diseases. It is not fully known what factor(s) regulates local basophil influx in tissues, and it has not been determined whether antigens belong in a panel of basophil chemoattractants. This study was designed to elucidate whether IgE- and high-affinity receptor for IgE (Fc{varepsilon}RI)-mediated stimulation can induce human basophil migration. The migration-inducing potency of an anti-Fc{varepsilon}RI {alpha}-chain mAb, CRA-1, was examined on human basophils. CRA-1 mAb elicited significant migration of basophils. The migration-inducing potency of this mAb was maximal at 100 ng ml–1, and CRA-1 mAb at 100 ng ml–1 attracted ~10% of total inoculated basophils above baseline levels after incubation for 2.5 h. Checkerboard analysis indicated that basophil migration induced by this mAb was mainly chemotactic and partially chemokinetic. An antigen, Der f 2, also induced migration of basophils from Der f-sensitive subjects. Basophils mixed with 1 ng ml–1 of CRA-1 mAb showed an exaggerated migration response to eotaxin, indicating that Fc{varepsilon}RI cross-linkage enhances basophil migration to other chemoattractants. Induction of basophil migration by IgE- and Fc{varepsilon}RI-cross-linking stimulation may, at least in part, explain the pathogenesis of local basophil accumulation clinically observed in allergic diseases such as asthma.

Keywords: allergy, antigen, chemotaxis, non-releaser

Transmitting editor: S. J. Galli


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M. Suzukawa, A. Komiya, M. Iikura, H. Nagase, C. Yoshimura-Uchiyama, H. Yamada, H. Kawasaki, K. Ohta, K. Matsushima, K. Hirai, et al.
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[Abstract] [Full Text] [PDF]


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