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International Immunology Advance Access originally published online on July 6, 2005
International Immunology 2005 17(8):973-982; doi:10.1093/intimm/dxh280
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© The Japanese Society for Immunology. 2005. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org

A negative regulatory element in the rabbit 3'IgH chromosomal region

Veronica Volgina1, Pi-Chen Yam and Katherine L. Knight

Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL 60153, USA
1 Present address: Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA

Correspondence to: K. L. Knight; E-mail: kknight{at}lumc.edu

Mouse and human IgH loci contain several 3'IgH enhancers. In rabbit, a single hs1,2 enhancer is located 3' of the distal germ line C{alpha} gene, C{alpha}13. We searched for additional regulatory elements in this region by using a luciferase reporter assay and nucleotide sequence analysis. Within 8 kb 3' of C{alpha}13, we identified a 1-kb fragment that negatively regulated the hs1,2 enhancement of the I{alpha} promoter. This negative regulatory element, C{alpha}-NRE, contains a conserved 300-bp region that is associated with 8 of the 13 germ line C{alpha} genes. This conserved region contains an E box that, by electrophoretic mobility shift assay, binds an E47-like protein. At the 5' end, C{alpha}-NRE also includes a 270-bp region with 20-bp repeats nearly identical to those 3' of mouse and human C{alpha} genes, and these repeats bind unidentified nuclear protein(s). C{alpha}-NRE appears to be a novel regulatory element that may contribute to the regulation of IgH gene expression.

Keywords: B cells, gene regulation, molecular biology, rabbit, transcription factors

Transmitting editor: W. Strober


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