International Immunology Advance Access originally published online on July 19, 2005
International Immunology 2005 17(8):1103-1115; doi:10.1093/intimm/dxh290
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MHC II+ CD45+ cells from synovium-rich tissues of normal rats: phenotype, comparison with macrophage and dendritic cell lineages and differentiation into mature dendritic cells in vitro
1 Arthritis Research Laboratory, Hanson Institute for Medical Research, Institute of Medical and Veterinary Science, Adelaide, South Australia
2 Rheumatology Unit, Eleanor Harrald Building, Level 4, Royal Adelaide Hospital, North Terrace, Adelaide, South Australia
3 Discipline of Microbiology and Immunology, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia
Correspondence to: L. G. Cleland; E-mail: lcleland{at}mail.rah.sa.gov.au
Synovial tissues are frequent sites of inflammatory disorders in which dendritic cells (DCs) may play an important role. This study examines potential antigen-presenting cells obtained from synovium-rich tissues (SRTs) by vascular perfusion of rat hind limbs with collagenase and further enzymatic digestion of the disarticulated hind paws in vitro. The three sub-populations of interest were: CD45+MHC IIhi, mainly CD11c+ and CD163–; CD45+MHC IIlo, mainly CD11c– and CD163+ and CD45+MHC II–, mainly CD11c– and CD163+. Expression of CD11c and CD163 correlated with ruffled cell-surface (CD11c+CD163–) and highly vacuolated cytoplasm (CD11c–CD163+), respectively. Culture of the CD45+CD163– sub-population in granulocyte macrophage colony-stimulating factor (GM-CSF) yielded CD45+MHC IIhi CD11c+CD163– cells with veiled morphology, while the large vacuolated cells that expressed CD163 resembled type A synoviocytes in both surface antigen phenotype and morphology. These results demonstrate that SRTs contain indeterminate cells that can differentiate into mature DCs in vitro in response to GM-CSF, plus mature synovial lining macrophages.
Keywords: animal model, antigen-presenting cells, cytokines, polyarthritis, synovial cells
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