International Immunology Advance Access originally published online on May 20, 2005
International Immunology 2005 17(7):857-867; doi:10.1093/intimm/dxh265
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Selection of stereotyped VH81X-µH chains via pre-B cell receptor early in ontogeny and their conservation in adults by marginal zone B cells
Department of Immune Regulation, Tokyo Medical and Dental University Graduate School, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan
Correspondence to: H. Karasuyama; E-mail: karasuyama.mbch{at}tmd.ac.jp
The pre-B cell receptor (preBCR) plays critical roles in early B cell differentiation. It has been shown that not all µH chains are capable of pairing with surrogate light (SL) chains to form preBCR. Here, we established a novel system to differentially identify two types of early pre-B cell populations in bone marrow and fetal liver of mice, one producing SL-pairing µH chains and the other producing SL-non-pairing µH chains. The former population accounted for 80% of all the early pre-B cells in adult bone marrow, while it accounted for only 20% of those in fetal liver. Comparison of the two types of pre-B cell populations in fetal liver revealed the structural difference between SL-pairing and -non-pairing µH chains encoded by the VH81X segment that was most frequently utilized in fetal liver pre-B cells but rarely expressed by B cells generated in adults. PreBCR played an important role in the positive selection of VH81X-µH chains carrying the characteristic sequences of the complementarity-determining region 3 with little or no nibbling or N nucleotide addition, leading to their predominance in neonatal splenic B cells. These fetal-type VH81X-µH chains were also detected in adult spleen, but almost exclusively in marginal zone (MZ) B cells in contrast to the adult-type VH81X-µH chains. This strongly suggests that neonatally generated and selected B cells expressing the stereotyped VH81X-µH chains are maintained in the adult MZ and could function as innate-like lymphocytes.
Keywords: B cell development, B cell repertoire, B cell subsets, immunoglobulin, fetal liver
Transmitting editor: M. Miyasaka
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