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International Immunology Advance Access originally published online on May 17, 2005
International Immunology 2005 17(6):671-676; doi:10.1093/intimm/dxh254
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© The Japanese Society for Immunology. 2005. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org

Immunosynapse formation coincides with rapid activation of NK cells by syngeneic T cells and correlates with clustering of MHC class I

Brian A. Rabinovich1, Jennifer Li2,3, Rose Hurren3 and Richard G. Miller2,3,4

1 Amgen Washington, 1201 Amgen Court West, Seattle, WA 98119, USA
2 Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
3 Ontario Cancer Institute, Division of Cell and Molecular Biology, Room 9-305, 610 University Avenue, Toronto, Ontario M5G 2M9, Canada
4 Department of Immunology, University of Toronto, Toronto, Ontario, Canada

Correspondence to: R. G. Miller; E-mail: miller{at}oci.utoronto.ca

T cells cultured for 3 h with antigen-presenting cells (APCs) stimulated syngeneic IL-2-activated NK cells as measured via a standard chromium-release assay. Discrete caps containing both TCR and MHC-I had formed on the surface of these activated T cells. When conjugates were formed between NK cells and these activated T cells, >80% of the contact sites were in the MHC-Idim region outside the TCR–MHC-I cap. Stimulation with phorbol myristate acetate plus Ionomycin, which bypasses the need for cell surface events during activation, did not induce either cap formation or NK cell activation. Further, the addition of the protein transport inhibitor Brefeldin A did not block activation of NK cells. MHC-I is the major inhibitory ligand recognized by NK cells. One possible mechanism for the activation of NK cells by TCR–MHC-I-capped T cells is that aggregation of MHC-I into one region leaves the remaining T cell surface denuded of ligands for NK-inhibitory receptors. As a test of this hypothesis, we aggregated MHC-I on T cells with plate-bound anti-MHC-I mAb. This treatment conferred upon the T cells the capacity to activate NK cells, suggesting that MHC-I clustering could contribute to the observed phenomenon.

Keywords: cytotoxicity, immunological synapse, lymphokine-activated killer cells, mice, T-lymphocytes

Transmitting editor: W. M. Yokoyama


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